Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain
| Autor: | Steven A. McCarroll, Aleksandrina Goeva, Laura Bortolin, Melissa Goldman, Fenna M. Krienen, Shuyu Wang, David Kulp, Sara A. Brumbaugh, Alec Wysoker, Heather de Rivera, Nolan Kamitaki, Arpiar Saunders, Evan Z. Macosko, Matthew A. Baum, James Nemesh, Elizabeth Bien |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Cell Population Striatum Biology Medium spiny neuron Brain Cell General Biochemistry Genetics and Molecular Biology Article Mice 03 medical and health sciences Glutamatergic Basal ganglia medicine Animals Cell Lineage Gene Regulatory Networks education Ion channel education.field_of_study Gene Expression Profiling Brain Gene Expression Regulation Developmental High-Throughput Nucleotide Sequencing Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Single-Cell Analysis Transcriptome Neuroscience |
| Popis: | The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells, sampled from nine regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals. Cross-region analysis of these 565 cell populations revealed features of brain organization, including a gene-expression module for synthesizing axonal and presynaptic components, patterns in the co-deployment of voltage-gated ion channels, functional distinctions among the cells of the vasculature and specialization of glutamatergic neurons across cortical regions. Systematic neuronal classifications for two complex basal ganglia nuclei and the striatum revealed a rare population of spiny projection neurons. This adult mouse brain cell atlas, accessible through interactive online software (DropViz), serves as a reference for development, disease, and evolution. |
| Databáze: | OpenAIRE |
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