Lens-Free Imaging as a Sensor for Dynamic Cell Viability Detection Using the Neutral Red Uptake Assay
| Autor: | Brian J. Nablo, Kiran Bhadriraju, Jong Muk Lee, Jungjoon Ahn, Darwin R. Reyes |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Neutral red
Quantitative imaging Chemistry Biochemistry (medical) Biomedical Engineering General Chemistry Article Biomaterials chemistry.chemical_compound medicine.anatomical_structure Supravital staining Lens (anatomy) medicine Standard protocol Biophysics Viability assay human activities health care economics and organizations |
| Zdroj: | ACS Appl Bio Mater |
| Popis: | Neutral red is a low-cost supravital stain for determining cell viability. The standard protocol relies on a destructive extraction process to release the accumulated dye for endpoint spectrophotometric quantification. We report a non-destructive, live-cell quantification of neutral red uptake using a compact lens-free system. Two light sources indentify the cell perimeter and quantify neutral red uptake. The quantification occurs during staining, thus eliminating the destructive extraction process and reducing assay time. Our system enables live quantification for continuous high-throughput screening of cell viability within confined spaces such as incubators. |
| Databáze: | OpenAIRE |
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