Progesterone inhibits cytokine/TNF-α production by porcine CL macrophages via the genomic progesterone receptor

Autor: S. Frandsen, Emily K. Tucker, John E. Gadsby, J. Chang, B. Celestino, Daniel H Poole
Rok vydání: 2019
Předmět:
endocrine system
medicine.medical_specialty
Swine
medicine.medical_treatment
Inhibitory postsynaptic potential
Andrology
03 medical and health sciences
0302 clinical medicine
Endocrinology
Food Animals
Internal medicine
Progesterone receptor
medicine
Animals
RNA
Messenger
Receptor
Cells
Cultured
Progesterone
Estrous cycle
Messenger RNA
030219 obstetrics & reproductive medicine
Dose-Response Relationship
Drug
Chemistry
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Macrophages
0402 animal and dairy science
04 agricultural and veterinary sciences
Genomics
040201 dairy & animal science
Cytokine
medicine.anatomical_structure
Gene Expression Regulation
Cytokines
Animal Science and Zoology
Tumor necrosis factor alpha
Receptors
Progesterone
Corpus luteum
hormones
hormone substitutes
and hormone antagonists
Zdroj: Domestic animal endocrinology. 72
ISSN: 1879-0054
Popis: In pigs, luteolytic sensitivity to PGF-2α (=LS) is delayed until d 13 of the estrous cycle. While the control of LS is unknown, it is temporally associated with macrophage (MAC; which secretes tumor necrosis factor [TNF]-α) infiltration into the corpora lutea (CL), and previous studies have shown that TNF-α induces LS in porcine luteal cells (LCs) in culture. This study was designed to explore the control of LS by CL macrophage (CL MAC)/TNF-α by progesterone (P4), and to examine the hypothesis that P4 acting via the genomic P4 receptor (PGR) inhibits CL MAC TNF-α and thus plays a key role in regulating LS during the pig estrous cycle. In experiment 1, the effects of LCs on CL MAC cytokine/TNF-α mRNA expression in co-culture were examined (MID cycle; ~d 7-12; no LS); results showed that LC was inhibitory to cytokine/TNF-α. In experiment 2, the effects of P4 or R5020 (PGR-agonist) on CL MAC cytokine/TNF-α mRNA expression were examined (MID cycle; ~d 7-12; no LS); results showed that both P4 and R5020 dose-dependently inhibited TNF-α. In experiment 3, CL MACs were isolated from CL at MID (~d 7-12; no LS) and LATE (~d 13-18; + LS) cycle, and TNF-α/PGR mRNA measured. Results indicated that while TNF-α mRNA was 4.2-fold greater in CL MACs from LATE vs MID cycle, PGR mRNA was 4.5-fold greater in CL MACs from MID vs LATE cycle. These data support our hypothesis and suggest that progesterone, acting via PGR, plays a critical physiological role in the control of TNF-α production by CL MACs and LS during the pig estrous cycle.
Databáze: OpenAIRE
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