Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo
| Autor: | Oksana B. Polivanova, Mikhail Yu. Cherednichenko, E. N. Baranova, Marat R. Khaliluev, Rima N. Kirakosyan, Ivan G. Tarakanov, Alla A. Shulgina, Elena A. Kalashnikova |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
clonal micropropagation spectral light composition morphogenesis Plant Science Horticulture 01 natural sciences SB1-1110 03 medical and health sciences Basal shoot Murashige and Skoog medium In vivo 030304 developmental biology chemistry.chemical_classification plant growth regulators (PGRs) 0303 health sciences light-emitting diodes (LED) Chemistry Plant culture Hydroxycinnamic acid In vitro Stevia rebaudiana Micropropagation Composition (visual arts) 010606 plant biology & botany Stevia rebaudiana Bertoni |
| Zdroj: | Horticulturae Volume 7 Issue 7 Horticulturae, Vol 7, Iss 195, p 195 (2021) |
| ISSN: | 2311-7524 |
| DOI: | 10.3390/horticulturae7070195 |
| Popis: | We investigated the influence of different conditions (light composition and plant growth regulators (PGRs) in culture media) on the morphophysiological parameters of Stevia rebaudiana Bertoni in vitro and in vivo. Both PGRs and the light spectra applied were found to significantly affect plant morphogenesis. During the micropropagation stage of S. rebaudiana, optimal growth, with a multiplication coefficient of 15, was obtained in an MS culture medium containing 2,4-epibrassinolide (Epin) and indole-3-acetic acid (IAA) at concentrations of 0.1 and 0.5 mg L−1, respectively. During the rooting stage, we found that the addition of 0.5 mg L−1 hydroxycinnamic acid (Zircon) to the MS medium led to an optimal root formation frequency of 85% and resulted in the formation of strong plants with well-developed leaf blades. Cultivation on media containing 0.1 mg L−1 Epin and 0.5 mg L−1 IAA and receiving coherent light irradiation on a weekly basis resulted in a 100% increase in the multiplication coefficient, better adventitious shoot growth, and a 33% increase in the number of leaves. S. rebaudiana microshoots, cultured on MS media containing 1.0 mg L−1 6-benzylaminopurine (BAP) and 0.5 mg L−1 IAA with red monochrome light treatments, increased the multiplication coefficient by 30% compared with controls (white light, media without PGRs). |
| Databáze: | OpenAIRE |
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