Characterization and expression of cell division cycle 2 (Cdc2) mRNA and protein during ovarian development of the giant tiger shrimp Penaeus monodon
| Autor: | Sittiruk Roytrakul, Narumon Phaonakrop, Sirawut Klinbunga, Mahattanee Phinyo, Virak Visudtiphole, Padermsak Jarayabhand |
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| Rok vydání: | 2013 |
| Předmět: |
Molecular Sequence Data
Ovary Cell Cycle Proteins Broodstock Mass Spectrometry Penaeus monodon Endocrinology Western blot Penaeidae medicine Animals Amino Acid Sequence RNA Messenger Phosphorylation Cyclin-dependent kinase 1 Messenger RNA biology medicine.diagnostic_test Base Sequence Reverse Transcriptase Polymerase Chain Reaction Gene Expression Regulation Developmental biology.organism_classification Molecular biology medicine.anatomical_structure Polyclonal antibodies biology.protein Animal Science and Zoology Female Vitellogenesis Sequence Alignment |
| Zdroj: | General and comparative endocrinology. 193 |
| ISSN: | 1095-6840 |
| Popis: | The meiotic maturation of oocytes is regulated by the maturation-promoting factor (MPF), a complex of Cdc2 (Cdk1) and Cyclin B. Here, the complete open reading frame (ORF) of Cdc2 in Penaeus monodon was characterized. PmCdc2 were 900bp in length corresponding to a polypeptide of 299 amino acids with the conserved Thr14, Tyr15 and Thr161 residues. Quantitative real-time PCR indicated that the expression level of PmCdc2 in wild intact broodstock was significantly increased in stages II (vitellogenic) and III (early cortical rod) ovaries relative to stage I (previtellogenic) ovaries and peaked in stage IV (mature) ovaries (P |
| Databáze: | OpenAIRE |
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