Knockdown of survivin expression by siRNAs enhances chemosensitivity of prostate cancer cells and attenuates its tumorigenicity
| Autor: | Mingquan Su, Yin Long, Yinye Miao, Qing Zhang, Hua Han, Jiayun Liu, Xiaoke Hao, Jian-Jun Shen |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Small interfering RNA Cell Survival Survivin Biophysics Mice Nude Tetrazolium Salts Antineoplastic Agents Apoptosis Biology Transfection Inhibitor of apoptosis Biochemistry Inhibitor of Apoptosis Proteins Mice Prostate cancer Cell Line Tumor medicine Animals Humans Gene Silencing RNA Messenger RNA Small Interfering Cell Proliferation Gene knockdown Formazans Cell growth Prostatic Neoplasms Cancer Genetic Therapy General Medicine medicine.disease Immunohistochemistry Xenograft Model Antitumor Assays Tumor Burden Androgens Cancer research Feasibility Studies RNA Interference Cisplatin Microtubule-Associated Proteins |
| Zdroj: | Acta Biochimica et Biophysica Sinica. 41:223-230 |
| ISSN: | 1672-9145 |
| DOI: | 10.1093/abbs/gmp005 |
| Popis: | Survivin, a member of inhibitor of apoptosis family protein, has become an attractive therapeutic target in cancer due to its selective expression in tumor cells and its important roles for tumor cell viability. Here, we show that vector-based small interfering RNAs (siRNAs) silenced survivin expression in prostate cancer cells, resulting in significantly reduced cell proliferation and enhanced apoptosis, and increased the sensitivity of prostate cancer cells (PC-3) to the apoptosis- inducing agent, platinol. Furthermore, PC-3 cells transfected with the siRNA-expressing vector showed lower tumor formation in nude mice xenografts in vivo. These results demonstrated that inhibition of survivin expression by siRNA attenuated the malignant phenotypes of prostate cancer cells, and may provide a novel approach for gene therapy of androgen-independent prostate cancer. |
| Databáze: | OpenAIRE |
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