Identification and Characterization of Two 'Sensory Neuron Membrane Proteins' (SNMPs) of the Desert Locust, Schistocerca gregaria (Orthoptera: Acrididae)
Autor: | Xingcong Jiang, Heinz Breer, Pablo Pregitzer, Ewald Grosse-Wilde, Jürgen Krieger |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
desert locust Arthropod Antennae sensory neuron membrane protein animal structures Sensory Receptor Cells Grasshoppers Biology Acrididae antenna 03 medical and health sciences 0302 clinical medicine Botany medicine Animals Desert locust Phylogeny Antenna (biology) Reverse Transcriptase Polymerase Chain Reaction fungi Membrane Proteins General Medicine biology.organism_classification Sensory neuron Receptors Pheromone Cell biology 030104 developmental biology medicine.anatomical_structure nervous system Insect Science Sex pheromone Insect Proteins Schistocerca Neuron in situ hybridization 030217 neurology & neurosurgery Locust Research Article olfaction |
Zdroj: | Journal of Insect Science |
ISSN: | 1536-2442 |
Popis: | Pheromone-responsive neurons of insects not only require specific receptors but in addition several auxiliary components, including the “sensory neuron membrane protein,” SNMP. Accordingly, SNMP is considered as a marker for neurons responding to pheromones. For the desert locust Schistocerca gregaria, it is known that the behavior, including aggregation behavior and courtship inhibition, is largely controlled by pheromones. However, little is known about pheromones, their receptors, and the pheromone-responsive cells in locusts. In this study, we have identified two SNMP subtypes, SNMP1 and SNMP2, and compared their phylogenetic relationship and primary structure motifs with SNMPs from other species. Both SNMPs were found in chemosensory tissues, especially the antennae. Employing double in situ hybridization, we identified and localized the SNMP-expressing cells in the antennae. Cells expressing SNMP1 were localized to sensilla trichodea but also to sensilla basiconica, which in locust respond to pheromones. One or a few cells express SNMP1 within the multineuron clusters from sensilla basiconica, whereas the SNMP2 subtype was expressed in cells surrounding the neuron clusters, possibly supporting cells. Based on the finding that SNMP1 is expressed in distinct neurons under chemosensory sensilla, it is conceivable that these cells may represent pheromone-responsive neurons of the desert locust. |
Databáze: | OpenAIRE |
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