Correlation of MYCN amplification with MCM7 protein expression in neuroblastomas: A chromogenic in situ hybridization study in paraffin sections
| Autor: | Shiu-Feng Huang, Shih-Feng Tsai, Bae-Li Hsi, Chao-Ping Yang, Jer-Nan Lin, Hsien-Yu Tsai, Iou-Jih Hung, Jeng-Chang Chen |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Adolescent Chromogenic in situ hybridization Cell Cycle Proteins Biology Pathology and Forensic Medicine Neuroblastoma Minichromosome Biomarkers Tumor medicine Humans Stage (cooking) Child CISH neoplasms In Situ Hybridization Oncogene Proteins N-Myc Proto-Oncogene Protein Paraffin Embedding medicine.diagnostic_test Gene Amplification Infant Newborn Infant Nuclear Proteins Anatomical pathology Minichromosome Maintenance Complex Component 7 medicine.disease Immunohistochemistry DNA-Binding Proteins Child Preschool Cancer research Female Fluorescence in situ hybridization |
| Zdroj: | Human Pathology. 35:1397-1403 |
| ISSN: | 0046-8177 |
| DOI: | 10.1016/j.humpath.2004.07.014 |
| Popis: | Amplification of the MYCN oncogene in neuroblastomas is generally associated with a more aggressive clinical course. Recently, 1 of the minichromosome maintenance proteins, MCM7, was found to be a direct target of the MYCN transcription factor in neuroblastoma. To confirm this correlation, chromogenic in situ hybridization (CISH) to detect MYCN amplification and immunohistochemical staining for MCM7 protein expression were performed on paraffin tissue sections of 26 neuroblastomas cases and of 4 recurrences of these tumors. Seven of the primary tumors showed MYCN amplification, and all were stage 3 or 4 tumors. Only 4 of these showed MCM7 overexpression. However, 11 primary tumors overexpressed MCM7. The 4 patients with MCM7 expression associated with MYCN amplification all died from the tumor. In contrast, the 7 patients with MCM7 overexpression but no MYCN amplification were all younger than 1 year of age and have shown good survival. This suggests that MCM7 overexpression by itself is not related to a poorer prognosis as is MYCN amplification. In addition, the 4 pairs of primary and recurrent tumors all showed changes in MCM7 expression from negative to positive, whereas none of them had MYCN amplification. This study showed that MCM7 overexpression is not necessarily correlated with MYCN amplification or an aggressive clinical course. Interpretation of the results of CISH was quite easy and straightforward because the preparations were viewed with an ordinary light microscope with good preservation of the tissue morphology. |
| Databáze: | OpenAIRE |
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