Cloning and Characterization of 13 Novel Transcripts and the Human RGS8 Gene from the 1q25 Region Encompassing the Hereditary Prostate Cancer (HPC1) Locus
| Autor: | Greg Landes, Tom I. Bonner, Heather W. Pinkett, Christiane M. Robbins, K W Klinger, Tim D. Connors, Andreas D. Baxevanis, Mezbah U. Faruque, Dietrich A. Stephan, John D. Carpten, Raman Sood, Jeffrey M. Trent, Izabela Makalowska, Christopher L. Graham, Kui Su, Sharon D. Morgenbesser |
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| Rok vydání: | 2001 |
| Předmět: |
Male
DNA Complementary Transcription Genetic Molecular Sequence Data Gene Expression Locus (genetics) Biology Contig Mapping Exon Gene mapping Neoplastic Syndromes Hereditary Genetics Animals Humans Genetic Predisposition to Disease Amino Acid Sequence Genomic organization Expressed Sequence Tags Expressed sequence tag tRNA Methyltransferases Contig Sequence Homology Amino Acid Genome Human Gene Expression Profiling Hyperparathyroidism Intron Prostatic Neoplasms food and beverages Jaw Neoplasms Rats Parathyroid Neoplasms Chromosomes Human Pair 1 Mutation RGS Proteins |
| Popis: | The aim of this study was to develop a saturated transcript map of the region encompassing the HPC1 locus to identify the susceptibility genes involved in hereditary prostate cancer (OMIM 176807) and hyperparathyroidism-jaw tumor syndrome (OMIM 145001). We previously reported the generation of a 6-Mb BAC/PAC contig of the candidate region and employed various strategies, such as database searching, exon-trapping, direct cDNA hybridization, and sample sequencing of BACs, to identify all potential transcripts. These efforts led to the identification and precise localization on the BAC contig of 59 transcripts representing 22 known genes and 37 potential transcripts represented by ESTs and exon traps. Here we report the detailed characterization of these ESTs into full-length transcript sequences, their expression pattern in various tissues, their genomic organization, and their homology to known genes. We have also identified an Alu insertion polymorphism in the intron of one of the transcripts. Overall, data on 13 novel transcripts and the human RGS8 gene (homologue of the rat RGS8 gene) are presented in this paper. Ten of the 13 novel transcripts are expressed in prostate tissue and represent positional candidates for HPC1. |
| Databáze: | OpenAIRE |
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