| Autor: |
C. Elijah, Allison Blomme, Olivia Harrison, Jianfa Bai, Jason Woodworth, Cassandra Jones, Elizabeth Poulsen-Porter, Chad Paulk, Jordan Gebhardt |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Journal of Swine Health and Production. 31:87-91 |
| ISSN: |
1537-209X |
| DOI: |
10.54846/jshap/1311 |
| Popis: |
This study evaluated sample processing methods and the presence of organic matter on detection of porcine epidemic diarrhea virus (PEDV) from environmental samples using real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Steel coupons were inoculated with PEDV and different types of organic material contamination. Surface samples were collected and processed in one of four ways: none, centrifugation, syringe filtration, or combination of centrifugation and syringe filtration, then submitted for PEDV qRT-PCR. There was a surface inoculation type by processing method interaction (P < .001) that impacted the sample cycle threshold value. Centrifugation resulted in the most consistent detection of PEDV RNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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