Proteomic fingerprinting of HIV-1-infected human monocyte-derived macrophages: a preliminary report
| Autor: | Courtney N. Schellpeper, Xiaoguang Luo, Kim A Carlson, Rong Fong Shen, Toni M. Biskup, Pawel Ciborowski, Howard E. Gendelman, Christopher J. Destache |
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| Rok vydání: | 2004 |
| Předmět: |
Lineage (genetic)
Proteome Immunology Protein Array Analysis Human immunodeficiency virus (HIV) HIV Infections Biology Proteomics medicine.disease_cause Peptide Mapping Gas Chromatography-Mass Spectrometry Virus Immune system Sequence Analysis Protein medicine Humans Immunology and Allergy Lymph node Cells Cultured Chromatography High Pressure Liquid Microglia Macrophages Hepatitis B medicine.anatomical_structure Neurology HIV-1 Neurology (clinical) Intracellular |
| Zdroj: | Journal of Neuroimmunology. 147:35-42 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/j.jneuroim.2003.10.039 |
| Popis: | Mononuclear phagocytes (MP; blood monocytes, alveolar, lymph node, and brain macrophages and microglia) are vehicles for dissemination and principle target cells for human immunodeficiency virus type 1 (HIV-1) infection. Notably, viral persistence in macrophages occurs despite ongoing phagocytic, intracellular killing, innate and adaptive immune responses. To assess potential pathways for how HIV-1 may bypass antiviral MP responses, we used proteomic tests to evaluate protein fingerprints of HIV-1-infected human monocyte-derived macrophages 7 days after viral infection. By using weak cation exchange chips, 58 proteins were found up- or down-regulated after HIV-1ADA infection. Several of these proteins were identified by microsequencing. It is probable that cellular proteins identified by proteomic fingerprinting could assist in unraveling how persistent viral infection occurs in MP lineage cells. Moreover, this evolving technology can be utilized to unravel changes in immune activities initiated by interactions between virus, environmental cues and drugs of abuse. |
| Databáze: | OpenAIRE |
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