Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat
Autor: | Jia Hong, Si-Yuan Song, Lei-Lei Lu, Xiao-Meng Zhai, Cheng-Jing Shan, Jun-Li Cao, Jia-Hao Dai, Li-Cai Zhang |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Neuroscience (miscellaneous) subcortex projection Biology medicine.disease_cause lcsh:RC321-571 lcsh:QM1-695 03 medical and health sciences Cellular and Molecular Neuroscience CSF-contacting nucleus 0302 clinical medicine Limbic system Cerebrospinal fluid limbic system medicine lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Original Research Basal forebrain Cholera toxin lcsh:Human anatomy Spinal cord Preoptic area 030104 developmental biology medicine.anatomical_structure nervous system retrograde trace Anatomy Nucleus Neuroscience 030217 neurology & neurosurgery Homeostasis |
Zdroj: | Frontiers in Neuroanatomy, Vol 14 (2020) Frontiers in Neuroanatomy |
ISSN: | 1662-5129 |
Popis: | Objective: To identify the novel projections received by the cerebrospinal fluid (CSF)-contacting nucleus from the subcortex and limbic system to understand the biological functions of the nucleus. Methods: The cholera toxin subunit B (CB), a retrograde tracer, was injected into the CSF-contacting nucleus in Sprague-Dawley rats. After 7-10 days, the surviving rats were perfused, and the whole brain and spinal cord were sliced for CB immunofluorescence detection. The CB-positive neurons in the subcortex and limbic system were observed under a fluorescence microscope, followed by 3D reconstructed with the imaris software. Results: CB-positive neurons were found in the basal forebrain, septum, periventricular organs, preoptic area, and amygdaloid structures. Five functional areas including 46 sub-regions sent projections to the CSF-contacting nucleus. However, the projections had different densities, ranging from sparse to moderate, to dense. Conclusions: According to the projections from the subcortex and limbic system, we hypothesize that the CSF-contacting nucleus participates in emotion, cognition, homeostasis regulation, visceral activity, pain, and addiction. In this study, we illustrate the novel projections from the subcortex and limbic system to the CSF-contacting nucleus, which underlies the diverse and complicated circuits of the nucleus in body regulations. |
Databáze: | OpenAIRE |
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