Pyridoxine regulates hair follicle development via the PI3K/Akt, Wnt and Notch signalling pathways in rex rabbits
| Autor: | Gongyan Liu, Gao Shuxia, Sun Haitao, Bai Liya, Chunyang Wang, Shu Li, Fuchang Li, Yongcui Zhang, Yanli Zhu, Guangmin Cheng |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
integumentary system Chemistry Wnt signaling pathway Pyridoxine Dermal papilla cell Bone morphogenetic protein Hair follicle SF1-1100 Hair follicle development Animal culture Rex rabbit medicine.anatomical_structure Endocrinology Food Animals Internal medicine medicine Animal Science and Zoology Folliculogenesis Original Research Article Protein kinase B PI3K/AKT/mTOR pathway medicine.drug |
| Zdroj: | Animal Nutrition Animal Nutrition, Vol 7, Iss 4, Pp 1162-1172 (2021) |
| ISSN: | 2405-6383 |
| Popis: | This study was conducted to evaluate the effect of pyridoxine on the development of hair follicles in Rex rabbits and the underlying molecular mechanism. Two hundred 3-month-old Rex rabbits were randomly divided into 5 groups and fed diets supplemented with 0, 5, 10, 20, or 40 mg/kg pyridoxine. The hair follicle density on the dorsal skin and the gene and protein expression levels of components of the phosphoinositide 3-kinase (PI3K)/protein kinase B (PKB or Akt), Wnt, Notch and bone morphogenetic protein (BMP) signalling pathways were measured. In addition, free hair follicles were isolated from Rex rabbits and cultured with pyridoxine in vitro to measure hair shaft growth. Furthermore, dermal papilla cells (DPC) were isolated from the skin of Rex rabbits and cultured with pyridoxine in vitro to measure the gene and protein expression levels of components of the PI3K/Akt, Wnt, Notch and BMP signalling pathways. The results showed that the addition of dietary pyridoxine significantly increased the total follicle density, secondary follicle density, and secondary-to-primary ratio (S/P, P |
| Databáze: | OpenAIRE |
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