Gene Structure and Functional Characterization of Growth Hormone in Dogfish, Squalus acanthias
| Autor: | Mayumi Oda, Tomoaki Goto, Shunsuke Moriyama, Tomohide Yamazaki, Masumi Nozaki, Hiroshi Kawauchi, Kiyoko Yamaguchi, Akiyoshi Takahashi, Noriko Amiya, Hiroshi Meguro, Masafumi Amano |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Pituitary gland Sequence analysis Molecular Sequence Data Biology Exon Species Specificity Squalus acanthias Internal medicine Complementary DNA medicine Consensus sequence Animals Cluster Analysis Gene family Amino Acid Sequence Cloning Molecular Peptide sequence Phylogeny DNA Primers Base Sequence Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis DNA Immunohistochemistry Gene Components medicine.anatomical_structure Endocrinology Liver Growth Hormone Pituitary Gland Animal Science and Zoology |
| Zdroj: | Zoological Science. 25:604-613 |
| ISSN: | 0289-0003 |
| Popis: | Dogfish (Squalus acanthias) growth hormone (GH) was identified by cDNA cloning and protein purification from the pituitary gland. Dogfish GH cDNA encoded a prehormone of 210 amino acids (aa). Sequence analysis of purified GH revealed that the prehormone is composed of a signal peptide of 27 aa and a mature protein of 183 aa. Dogfish GH showed 94% sequence identity with blue shark GH, and also showed 37-66%, 26%, and 48-67% sequence identity with GH from osteichtyes, an agnathan, and tetrapods. The site of production was identified through immunocytochemistry to be cells of the proximal pars distalis of the pituitary gland. Dogfish GH stimulates both insulin-like growth factor-I and II mRNA levels in dogfish liver in vitro. The dogfish GH gene consisted of five exons and four introns, the same as in lamprey, teleosts such as cypriniforms and siluriforms, and tetrapods. The 5'-flanking region within 1082 bp of the transcription start site contained consensus sequences for the TATA box, Pit-1/GHF-1, CRE, TRE, and ERE. These results show that the endocrine mechanism for growth stimulation by the GH-IGF axis was established at an early stage of vertebrate evolution, and that the 5-exon-type gene organization might reflect the structure of the ancestral gene for the GH gene family. |
| Databáze: | OpenAIRE |
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