Purified Human Skeletal Muscle-Derived Stem Cells Enhance the Repair and Regeneration in the Damaged Urethra
| Autor: | Nobuyuki Nakajima, Maki Hirata, Akio Hoshi, Tetsuro Tamaki, Masahiro Nitta, Toshiro Terachi, Shuichi Soeda |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pathology Time Factors medicine.medical_treatment 030232 urology & nephrology Antigens CD34 Cell Separation 0302 clinical medicine Medicine Angiogenic Proteins Cells Cultured Prostatectomy Integrin beta1 Stem Cells Anatomy Middle Aged Flow Cytometry medicine.anatomical_structure Phenotype Cytokines Stem cell medicine.medical_specialty 03 medical and health sciences Rats Nude Urethra Paracrine Communication Pressure Animals Humans Regeneration Muscle Skeletal Aged Cell Proliferation Transplantation Wound Healing business.industry Cell growth Regeneration (biology) Skeletal muscle Recovery of Function medicine.disease Rats Inbred F344 030104 developmental biology Erectile dysfunction Leukocyte Common Antigens Wound healing business Stem Cell Transplantation |
| Zdroj: | Transplantation. 101(10) |
| ISSN: | 1534-6080 |
| Popis: | Postoperative damage of the urethral rhabdosphincter and nerve-vascular networks is a major complication of radical prostatectomy and generally causes incontinence and/or erectile dysfunction. The human skeletal muscle-derived stem cells, which have a synchronized reconstitution capacity of muscle-nerve-blood vessel units, were applied to this damage.Cells were enzymatically extracted from the human skeletal muscle, sorted using flow cytometry as CD34/45 (Sk-34) and CD29/34/45 (Sk-DN/29) fractions, and separately cultured/expanded in appropriate conditions within 2 weeks. Urethral damage was induced by manually removing one third of the wall of the muscle layer in nude rats. A mixture of expanded Sk-34 and Sk-DN/29 cells was applied on the damaged portion for the cell transplantation (CT) group. The same amount of media was used for the non-CT (NT) group. Urethral pressure profile was evaluated via electrical stimulation to assess functional recovery. Cell engraftments and differentiations were detected using immunohistochemistry and immunoelectron microscopy. Expression of angiogenic cytokines was also analyzed using reverse transcriptase-polymerase chain reaction and protein array.At 6 weeks after transplantation, the CT group showed a significantly higher functional recovery than the NT group (70.2% and 39.1%, respectively; P0.05). Histological analysis revealed that the transplanted human cells differentiated into skeletal muscle fibers, nerve-related Schwann cells, perineuriums, and vascular pericytes. Active paracrine angiogenic cytokines in the mixed cells were also detected with enhanced vascular formation in vivo.The transplantation of Sk-34 and Sk-DN/29 cells is potentially useful for the reconstitution of postoperative damage of the urethral rhabdosphincter and nerve-vascular networks. |
| Databáze: | OpenAIRE |
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