Development of a Highly Sensitive and Specific Blastomycosis Antibody Enzyme Immunoassay Using Blastomyces dermatitidis Surface Protein BAD-1
| Autor: | Thomas E. Davis, L. Joseph Wheat, Melinda Smedema, Chadi A. Hage, Sarah M. Richer, Patricia Connolly, Bruce S. Klein, Diane S. Leland, T. Tristan Brandhorst, Michelle Durkin |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Antigens Fungal Clinical Biochemistry Immunology Enzyme-Linked Immunosorbent Assay Biology Sensitivity and Specificity Blastomycosis Histoplasmosis Microbiology Serology Diagnostic Laboratory Immunology medicine Humans Immunology and Allergy Antibodies Fungal Blastomyces medicine.diagnostic_test Clinical Laboratory Techniques Blastomyces dermatitidis Reproducibility of Results Complement fixation test medicine.disease biology.organism_classification Immunodiffusion Immunoassay |
| Zdroj: | Clinical and Vaccine Immunology. 21:143-146 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cvi.00597-13 |
| Popis: | Serologic tests for antibodies toBlastomyces dermatitidisare not thought to be useful for the diagnosis of blastomycosis, in part due to the low sensitivity of immunodiffusion and complement fixation. Earlier studies have shown that the enzyme immunoassay improves the sensitivity of antibody detection for the diagnosis of blastomycosis. Microplates coated with theB. dermatitidissurface protein BAD-1 were used for testing sera from patients with proven blastomycosis or histoplasmosis and controls. Semiquantification was accomplished by using standards containing human anti-B. dermatitidisantibodies. The antibodies were detected in 87.8% of the patients with blastomycosis by the enzyme immunoassay compared to 15.0% by immunodiffusion. The specificities were 99.2% for patients with nonfungal infections and healthy subjects and 94.0% for patients with histoplasmosis. The results were highly reproducible on repeat testing. When combined with antigen testing, antibody testing improved the sensitivity from 87.8% to 97.6%. Enzyme immunoassay detection of antibodies against BAD-1 is highly specific, has greatly improved sensitivity over immunodiffusion, and may identify cases with negative results by antigen testing. This assay has the potential to aid in the diagnosis of blastomycosis. |
| Databáze: | OpenAIRE |
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