Floral nectar of the obligate outcrossing Canavalia gladiata (Jacq.) DC. (Fabaceae) contains only one predominant protein, a class III acidic chitinase
| Autor: | Jiang-Yu Fang, Richard I. Milne, Hong-Guang Zha, Xue-Long Ma, Hong-Xia Zhou |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Plant Nectar Canavalia gladiata media_common.quotation_subject Nectarin Outcrossing Plant Science Insect Self-incompatibility 03 medical and health sciences Pollinator Floral nectar Botany Nectar Ecology Evolution Behavior and Systematics media_common Plant Proteins biology Obligate Chitinases food and beverages General Medicine Fabaceae biology.organism_classification Acidic class III chitinase Canavalia 030104 developmental biology Chitinase biology.protein |
| Zdroj: | Ma, X L, Milne, R, Zhou, H, Fang, J-Y & Zha, H-G 2017, ' Floral nectar of the obligate outcrossing Canavalia gladiata (Jacq.) DC. (Fabaceae) contains only one predominant protein, a class III acidic chitinase ', Plant Biology, vol. 19, no. 5, pp. 749-759 . https://doi.org/10.1111/plb.12583, https://doi.org/10.1111/plb.12583 |
| ISSN: | 1438-8677 |
| Popis: | Floral nectar can affect the fitness of insect-pollinated plants, through both attraction and manipulation of pollinators. Self-incompatible insect-pollinated plants receive more insect visits than their self-compatible relatives, and the nectar of such species might face more risk to be infested by the pathogens carried by pollinators than self-compatible plants. Proteins in nectar (nectarins) play an important role in the protection of nectar, but little is known regarding nectarins in self-incompatible species. The nectarins from a self-incompatible and insect-pollinated leguminous crop, Canavalia gladiata, were separated using two-dimensional electrophoresis and analysed using mass spectrometry. The predominant nectarin gene was cloned and the gene expression pattern was investigated by quantitative real-time PCR. Chitinolytic activity in the nectar was tested with different substrates. The C. gladiata nectar proteome only has one predominant nectarin, an acidic class III chitinases (CaChi3). The full-length CaChi3 gene was cloned, coding for a protein of 298 amino acids with a predicted signal peptide. CaChi3 has high similarity to members of the class III chitinase group, whose evolution has been dominated by purifying selection. CaChi3 was expressed in both nectary and leaves. CaChi3 showed thermostable chitinolytic activity by glycol-chitin zymography or using fluorogenic substrate but no lysozyme activity. Chitinase might be a critical proteinaceous component in nectar. The extremely simple nectar proteome in C. gladiata disproves a hypothesis that self-incompatible species always have more complex nectar proteomes. The accessibility of nectar might be a significant determinant of the evolutionary pressure to develop nectar defense mechanisms. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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