Small Buccal Fat Pad Cells Have High Osteogenic Differentiation Potential
| Autor: | Yoshinao Oki, Yoko Tamura, Morio Tonogi, Tomohiko Kazama, Niina Tsurumachi, Koichiro Kano, Masaki J. Honda, Taro Matsumoto, Keitaro Isokawa, Daisuke Akita, Taku Toriumi, Noriyoshi Shimizu |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Stromal cell Cellular differentiation Human Embryonic Stem Cells Biomedical Engineering Medicine (miscellaneous) Adipose tissue Bioengineering Cell Separation Real-Time Polymerase Chain Reaction Fluorescence Colony-Forming Units Assay 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Osteogenesis Adipocyte Adipocytes Humans Cell Shape Cells Cultured Cell Proliferation Cell Size Mouth Adipogenesis Gene Expression Profiling Mesenchymal stem cell Cell Differentiation 030206 dentistry Cell Dedifferentiation Fibroblasts Flow Cytometry Embryonic stem cell Cell biology chemistry Biochemistry Adipose Tissue 030220 oncology & carcinogenesis Alkaline phosphatase Female |
| Zdroj: | Tissue engineering. Part C, Methods. 22(3) |
| ISSN: | 1937-3392 |
| Popis: | Dedifferentiated fat (DFAT) cells derived from mature adipocytes have mesenchymal stem cells' (MSCs) characteristics. Generally, mature adipocytes are 60-110 μm in diameter; however, association between adipocyte size and dedifferentiation efficiency is still unknown. This study, therefore, investigated the dedifferentiation efficiency of adipocytes based on cell diameter. Buccal fat pad was harvested from five human donors and dissociated by collagenase digestion. After exclusion of unwanted stromal cells by centrifugation, floating adipocytes were collected and their size distribution was analyzed. The floating adipocytes were then separated into two groups depending on cell size using 40- and 100-μm nylon mesh filters: cell diameters less than 40 μm (small adipocytes: S-adipocytes) and cell diameters of 40-100 μm (large adipocytes: L-adipocytes). Finally, we evaluated the efficiency of adipocyte dedifferentiation and then characterized the resultant DFAT cells. The S-adipocytes showed a higher capacity to dedifferentiate into DFAT cells (S-DFAT cells) compared to the L-adipocytes (L-DFAT cells). The S-DFAT cells also showed a relatively higher proportion of CD146-positive cells than L-DFAT cells, and exhibited more osteogenic differentiation ability based on the alkaline phosphatase activity and amount of calcium deposition. These results suggested that the S- and L-DFAT cells had distinct characteristics, and that the higher dedifferentiation potential of S-adipocytes compared to L-adipocytes gives the former group an advantage in yielding DFAT cells. |
| Databáze: | OpenAIRE |
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