The effects of repeated automated plasmapheresis in goats (Capra hircus) in response to vaccination with purified influenza hemagglutinin proteins
| Autor: | Willie D. Taylor, Nathaniel Powell, Kristin A. Isenberg, David E. Wentworth, James L. Weed, Wei Song, George W. Lathrop, Thomas Rowe, Gregory Langham, Xiyan Xu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Influenza vaccine medicine.medical_treatment 030106 microbiology lcsh:Medicine Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Antibodies Viral Virus 03 medical and health sciences medicine Capra hircus Animals lcsh:Science Multidisciplinary biology business.industry Goats Viral Vaccine lcsh:R Vaccination Plasmapheresis Virology Influenza Vaccines biology.protein lcsh:Q Antibody business |
| Zdroj: | PLoS ONE, Vol 13, Iss 6, p e0195903 (2018) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0195903 |
| Popis: | Seasonal influenza is a contagious respiratory illness that annually affects millions of people worldwide. To identify currently circulating influenza virus subtypes, the Centers for Disease Control and Prevention's International Reagent Resource distributes the World Health Organization (WHO) influenza reagent kits, which are used globally by testing laboratories for influenza surveillance. The data generated by the kits aid in strain selection for the influenza vaccine each season. The use of animals to produce high quality and quantities of antibodies is critical to the production of these kits. In this study, we assessed the effects and efficacy of repeated sampling from automated plasmapheresis in goats. Analysis of blood samples demonstrated that repeated automated plasmapheresis procedures did not adversely affect the immediate or long-term health of goats. Further, our results indicate that repeated plasmapheresis in goats was capable of generating 2 liters of antibody-rich plasma per goat per week. This volume is sufficient to produce enough WHO influenza kits to conduct over 1 million tests. Thus, we have shown that the rapid production of plasma in goats can positively impact the public health preparedness and response to influenza. |
| Databáze: | OpenAIRE |
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