Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles
| Autor: | Till Matzat, Frank Schnorrer, Wilko Backer, Julia Sauerwald, Stefan Luschnig |
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| Přispěvatelé: | University of Münster, Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011), European Project: 310939,EC:FP7:ERC,ERC-2012-StG_20111109,FLIGHT MUSCLE(2013), Westfälische Wilhelms-Universität Münster = University of Münster (WWU) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
matrix metalloproteinase tube morphogenesis QH301-705.5 [SDV]Life Sciences [q-bio] Ramification (botany) Science Matrix (biology) Biology Matrix metalloproteinase Tracheal tube General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Live cell imaging Laminin tracheal development Biology (General) [SDV.BDD]Life Sciences [q-bio]/Development Biology General Immunology and Microbiology General Neuroscience General Medicine respiratory system Cell biology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology 030104 developmental biology Tube morphogenesis biology.protein Medicine muscle development branching morphogenesis flight muscle Developmental biology 030217 neurology & neurosurgery |
| Zdroj: | eLife, Vol 8 (2019) eLife eLife, eLife Sciences Publication, 2019, 8, pp.e48857. ⟨10.7554/eLife.48857⟩ eLife, eLife Sciences Publication, 2019, 8, ⟨10.7554/eLife.48857⟩ eLife, 2019, 8, pp.e48857. ⟨10.7554/eLife.48857⟩ |
| ISSN: | 2050-084X |
| Popis: | International audience; Tubular networks like the vasculature extend branches throughout animal bodies, but how developing vessels interact with and invade tissues is not well understood. We investigated the underlying mechanisms using the developing tracheal tube network of Drosophila indirect flight muscles (IFMs) as a model. Live imaging revealed that tracheal sprouts invade IFMs directionally with growth-cone-like structures at branch tips. Ramification inside IFMs proceeds until tracheal branches fill the myotube. However, individual tracheal cells occupy largely separate territories, possibly mediated by cell-cell repulsion. Matrix metalloproteinase 1 (MMP1) is required in tracheal cells for normal invasion speed and for the dynamic organization of growth-cone-like branch tips. MMP1 remodels the CollagenIV-containing matrix around branch tips, which show differential matrix composition with low CollagenIV levels, while Laminin is present along tracheal branches. Thus, tracheal-derived MMP1 sustains branch invasion by modulating the dynamic behavior of sprouting branches as well as properties of the surrounding matrix. |
| Databáze: | OpenAIRE |
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