Intraperitoneal administration of a novel chimeric immunogen (rOP) elicits IFN-γ and IL-12p70 protective immune response in BALB/c mice against virulent Brucella
| Autor: | H.S. Murali, Ganesh Tadepalli, Balakrishna Konduru, Harsh Vardhan Batra |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Immunogen Proliferation index medicine.medical_treatment Lipoproteins Recombinant Fusion Proteins Immunology Brucella Vaccine Brucella abortus Biology Brucellosis BALB/c Microbiology 03 medical and health sciences Interferon-gamma Mice Immune system Antigen medicine Escherichia coli Immunology and Allergy Animals Antigens Bacterial Mice Inbred BALB C Virulence Th1 Cells biology.organism_classification Interleukin-12 eye diseases 030104 developmental biology Polyclonal antibodies Periplasmic Binding Proteins Vaccines Subunit biology.protein Immunization Antibody Adjuvant Injections Intraperitoneal Bacterial Outer Membrane Proteins |
| Zdroj: | Immunology letters. 192 |
| ISSN: | 1879-0542 |
| Popis: | Recombinant engineering of immunologically active chimeric protein consisting of Omp19 and P39 domains of B. abortus (rOP), was purified under denaturing conditions upon expression in E. coli BL21 (DE3) and refolded to dynamic form. The immuno-protective efficacy of rOP was evaluated by challenging the BALB/c mice intraperitoneally (I.P) with the infective species of Brucella in the absence or presence of adjuvants, such as Aluminum hydroxide gel (Al), or Freund's Complete Adjuvant (FCA)/Incomplete Freund's Adjuvant (IFA). Surprisingly, after second boosting, mice received rOP per se were found to be immunogenic in terms of IgG response with the dominant expression of IgG2a and significant IFN-γ by splenic T cells, suggesting that rOP is a strong inducer of anti-Brucella immunity. The resulted anti-rOP antibodies recognized native Omp19 and P39 among species of Brucella with distinct double bands and single band against chimera in immunoblotting. An enhanced and comparable antibody response with varied IgG isotype combinations were noticed in the mice primed and boosted with rOP in adjuvants. However, rOP+FCA/IFA formulation was found to be the most effective in lymphocyte recall assays at inducing significant (P |
| Databáze: | OpenAIRE |
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