Deterioration of Tolerance to Hydrophobic Organic Solvents in a Toluene-Tolerant Strain of Pseudomonas putida under the Conditions Lowering Aerobic Respiration
| Autor: | Kasumi Noguchi, Norihiko Tsukagoshi, Rikizo Aono |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
biology
Cellular respiration Organic Chemistry chemistry.chemical_element General Medicine biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Toluene Electron transport chain Oxygen Pseudomonas putida Analytical Chemistry Solvent chemistry.chemical_compound chemistry Sodium azide Aerobie Molecular Biology Biotechnology Nuclear chemistry |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 63(8) |
| ISSN: | 0916-8451 |
| Popis: | The growth curve (increase in the number of viable cells) of a toluene-tolerant strain Pseudomonas putida Px51T was not reproducible in the presence of harmful organic solvents, such as p-xylene and toluene. The survival often fluctuated the during late exponential phase of growth. The repetitive growth was obtained by maintaining pO2 20-40% (v/v) in the culture flask. However, even under these aerobic conditions, the cells starved for a carbon source were killed by exposure to harmful solvents. The tolerance to organic solvents was lowered greatly by treatment with a proton conductor, carbonyl cyanide m-chlorophenylhydrazone (CCCP), or an electron transport chain inhibitor, sodium azide. Px51T treated with CCCP lost tolerance to a wide variety of organic solvents with log P ow of 2.6-4.2, which the organism usually tolerates. These results indicate that the solvent tolerance of Px51T depends upon on energy produced by aerobic respiration. |
| Databáze: | OpenAIRE |
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