HLA class II, MICA and PRL gene polymorphisms: the common contribution to the systemic lupus erythematosus development in Czech population

Autor: D. Tegzová, Markéta Fojtíková, Pavlina Cejkova, Satu Pešičková, Marie Cerna, P. Novota
Přispěvatelé: Institute of Rheumatology, Department of the General Biology and Genetics, Third Faculty of Medicine, Charles University [Prague] (CU), Department of Anthropology and Human Genetics, Faculty of Science
Rok vydání: 2010
Předmět:
Adult
Male
Linkage disequilibrium
Immunology
Population
Human leukocyte antigen
Gene
Polymerase Chain Reaction
Polymorphism
Single Nucleotide
Linkage Disequilibrium
03 medical and health sciences
Systemic lupus erythematosus
0302 clinical medicine
Gene Frequency
Rheumatology
Polymorphism (computer science)
Immunogenetics
Humans
Lupus Erythematosus
Systemic
Immunology and Allergy
SNP
Medicine
Genetic Predisposition to Disease
Polymorphism
Allele
skin and connective tissue diseases
10. No inequality
education
HLA-DRB1
Czech Republic
030203 arthritis & rheumatology
education.field_of_study
Polymorphism
Genetic
business.industry
Histocompatibility Antigens Class I
Haplotype
Histocompatibility Antigens Class II
Middle Aged
Prolactin
3. Good health
Haplotypes
Female
business
Polymorphism
Restriction Fragment Length
030215 immunology
Zdroj: ResearcherID
Rheumatology International
Rheumatology International, Springer Verlag, 2010, 31 (9), pp.1195-1201. ⟨10.1007/s00296-010-1431-4⟩
ISSN: 1437-160X
0172-8172
DOI: 10.1007/s00296-010-1431-4
Popis: International audience; The genetic components contribute to the systemic lupus erythematosus development. This study for the first time determined the distribution of the polymorphisms and linkage disequilibrium in HLA class II, MICA and PRL gene among patients suffering from SLE and healthy Czech individuals. DNA was obtained from the peripheral blood cells of 123 SLE patients and 96 healthy people. Allele variants of the HLA class II, MICA transmembrane polymorphism and PRL extrapituitary promoter −1149G/T SNP were detected using the sequence-specific primers analysis, PCR-fragment analysis and PCR-RFLP, respectively. In Czech population, only DRB1*03-DQB1*0201 haplotype is significantly associated with increased risk for SLE development: the frequency in SLE group was 44.7% in comparison with 15.2% in controls
Databáze: OpenAIRE
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