ROS-Responsive Nanoparticle as a Berberine Carrier for OHC-Targeted Therapy of Noise-Induced Hearing Loss

Autor: Yalan Li, Wei Zhuang, Konduru Naveena, Xi Shi, Ting Li, Xuanyi Li, Shiwei Qiu, Yuehua Qiao, Zhengzhong Han, Zeqi Zhao, Hongmei Liu, Guanxiong Lei
Rok vydání: 2021
Předmět:
0301 basic medicine
Materials science
Antioxidant
Berberine
Hearing loss
Surface Properties
medicine.medical_treatment
Guinea Pigs
Inflammation
Antioxidants
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
otorhinolaryngologic diseases
medicine
Evoked Potentials
Auditory
Brain Stem
Animals
General Materials Science
Particle Size
chemistry.chemical_classification
Reactive oxygen species
Drug Carriers
Molecular Structure
Anti-Inflammatory Agents
Non-Steroidal
medicine.disease
Disease Models
Animal
Hair Cells
Auditory
Outer
030104 developmental biology
medicine.anatomical_structure
chemistry
Hearing Loss
Noise-Induced
Drug delivery
Biophysics
Nanoparticles
sense organs
Hair cell
medicine.symptom
Reactive Oxygen Species
030217 neurology & neurosurgery
Noise-induced hearing loss
Zdroj: ACS applied materialsinterfaces. 13(6)
ISSN: 1944-8252
Popis: Overproduction of reactive oxygen species (ROS) and inflammation are two key pathogeneses of noise-induced hearing loss (NIHL), which leads to outer hair cell (OHC) damage and hearing loss. In this work, we successfully developed ROS-responsive nanoparticles as berberine (BBR) carriers (PL-PPS/BBR) for OHC-targeted therapy of NIHL: Prestin-targeting peptide 2 (PrTP2)-modified nanoparticles (PL-PPS/BBR), which effectively accumulated in OHC areas, and poly(propylene sulfide)120 (PPS120), which scavenged ROS and converted to poly(propylene sulfoxide)120 in a ROS environment to disintegrate and provoke the rapid release of BBR with anti-inflammatory and antioxidant effects. In this study, satisfactory anti-inflammatory and antioxidant effects of PL-PPS/BBR were confirmed. Immunofluorescence and scanning electron microscopy (SEM) images showed that PL-PPS/BBR effectively accumulated in OHCs and protected the morphological integrity of OHCs. The auditory brainstem response (ABR) results demonstrated that PL-PPS/BBR significantly improved hearing in NIHL guinea pigs after noise exposure. This work suggested that PL-PPS/BBR may be a new potential treatment for noise-associated injury with clinical application.
Databáze: OpenAIRE
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