GPE Promotes the Proliferation and Migration of Mouse Embryonic Neural Stem Cells and Their Progeny In Vitro
| Autor: | Pablo Devesa, Cristina Almengló, Jesús Devesa, Víctor M. Arce |
|---|---|
| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Fisioloxía |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway N-methyl-d-aspartate (NMDA) Neural regeneration Mice 0302 clinical medicine Neural Stem Cells Cell Movement Brain injury Extracellular Signal-Regulated MAP Kinases Cells Cultured Spectroscopy Kinase General Medicine Neural stem cell GH Computer Science Applications Cell biology ERK Biochemistry Signal transduction neural regeneration Oligopeptides Signal Transduction Neurogenesis GPE PI3K/Akt neural stem cells brain injury Biology Article Catalysis Inorganic Chemistry 03 medical and health sciences Extracellular Animals Physical and Theoretical Chemistry Molecular Biology Cell Proliferation Neural stem cells Cell growth Organic Chemistry Embryonic stem cell In vitro Mice Inbred C57BL 030104 developmental biology Growth Hormone Phosphatidylinositol 3-Kinase Proto-Oncogene Proteins c-akt 030217 neurology & neurosurgery |
| Zdroj: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) International Journal of Molecular Sciences; Volume 18; Issue 6; Pages: 1280 International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | This study was designed to investigate a possible role of the N-terminal tripeptide of insulin-like growth factor-1 (IGF-I), Gly-Pro-Glu (GPE), physiologically generated in neurons following IGF-I-specific cleavage, in promoting neural regeneration after an injury. Primary cultures of mouse neural stem cells (NSCs), obtained from 13.5 Days post-conception (dpc) mouse embryos, were challenged with either GPE, growth hormone (GH), or GPE + GH and the effects on cell proliferation, migration, and survival were evaluated both under basal conditions and in response to a wound healing assay. The cellular pathways activated by GPE were also investigated by using specific chemical inhibitors. The results of the study indicate that GPE treatment promotes the proliferation and the migration of neural stem cells in vitro through a mechanism that involves the activation of extracellular signal-regulated kinase (ERK) and phosphoinositide 3-kinase PI3K-Akt pathways. Intriguingly, both GPE effects and the signaling pathways activated were similar to those observed after GH treatment. Based upon the results obtained from this study, GPE, as well as GH, may be useful in promoting neural protection and/or regeneration after an injury SI |
| Databáze: | OpenAIRE |
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