Transportation and Routine Veterinary Interventions Alter Immune Function in the Dog
| Autor: | Yan Zhang, Catherine E. Hagan, Gabrielle N. Melillo, Rachel Halpin, Amy E. DeClue, Juliana Amorim, Cynthia Friedman Cowan, Sandra M. Axiak-Bechtel |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Veterinary medicine Neutrophils 040301 veterinary sciences medicine.medical_treatment Transportation Peripheral blood mononuclear cell 0403 veterinary science Dogs Immune system Stress Physiological Animals Medicine Lymphocytes Small Animals Physical Examination Toll-like receptor business.industry Monocyte 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science medicine.anatomical_structure Cytokine Peripheral blood lymphocyte Female business Hypothalamic–pituitary–adrenal axis Glucocorticoid medicine.drug |
| Zdroj: | Topics in Companion Animal Medicine. 39:100408 |
| ISSN: | 1938-9736 |
| DOI: | 10.1016/j.tcam.2020.100408 |
| Popis: | Rapid activation of the hypothalamic pituitary adrenal axis and the sympathetic nervous system are hallmarks of the acute stress response and these systems interact with the immune system by signaling though glucocorticoid and adrenergic receptors on immune cells. There is limited information about the effect of these physiologic responses on immunologic parameters of pet dogs enrolled in clinical studies. The objective of this study was to evaluate how travel, instrumentation, and hospitalization alter immunologic parameters in pet dogs. Blood was collected from healthy dogs in a home environment and from healthy dogs at the time of presentation to the hospital and after instrumentation and 24 hours of hospitalization. We found that lipopolysaccharide (LPS)-induced downregulation of toll like receptor 4 (TLR4) was blunted in dogs exposed to stress. Neutrophil and monocyte major histocompatibility complex class II (MHCII) expression increased after transportation to the veterinary hospital but then became similar to that of the control dogs at the end of hospitalization. Peripheral blood mononuclear cell cytotoxicity function was blunted in dogs exposed to the stress of transportation as well as hospitalization. Neutrophil apoptosis was greater in dogs exposed to stress compared to controls although this effect significantly decreased after hospitalization stress. Conversely, stress did not alter induced or spontaneous cytokine production from leukocytes, neutrophil or monocyte expression of TLR4, LPS-induced downregulation of monocyte TLR4, LPS-induced neutrophil and monocyte expression of MHCII or peripheral blood lymphocyte phenotype. Transportation and instrumentation/hospitalization stress should be considered when interpreting immunologic studies in pet dogs. |
| Databáze: | OpenAIRE |
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