Polymorphism and expression of the tumor necrosis factor receptor II gene in cows infected with the bovine leukemia virus
| Autor: | B. Bojarojc-Nosowicz, Alicja Stachura, Paweł Brym, E. Kaczmarczyk |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system viruses chemical and pharmacologic phenomena Single-nucleotide polymorphism 03 medical and health sciences 0302 clinical medicine Gene expression Leukemia Virus Bovine Animals Receptors Tumor Necrosis Factor Type II RNA Messenger Gene Regulation of gene expression Polymorphism Genetic General Veterinary Bovine leukemia virus biology hemic and immune systems General Medicine respiratory system Enzootic Bovine Leukosis biology.organism_classification Molecular biology 030104 developmental biology Gene Expression Regulation Cattle Gene polymorphism Tumor necrosis factor receptor 2 030215 immunology |
| Zdroj: | Polish journal of veterinary sciences. 19(1) |
| ISSN: | 1505-1773 |
| Popis: | A single T>C nucleotide polymorphism (rs42686850) of bovine tumor necrosis factor receptor type II gene (TNF-RII) is located within a sequence with allele-specific affinity to bind E2F transcription factors, considered pivotal in the regulation of cell cycle and cell proliferation. The objective of the study was to determine the effect of this SNP and BLV infection on the TNF-RII gene expression at the mRNA and protein levels in peripheral blood mononuclear cells (PBMC). We noted that analyzed TNF-RII gene polymorphism influenced the expression of the TNF-RII gene at the mRNA level but only in BLV-positive cows. Concurrently, no statistically significant association was found between gene polymorphism and TNF-RII expression at the protein level. However, we found a significant effect of BLV infection status on the amount of TNF-RII mRNA and the percentage of PBMC expressing TNF-RII. These results show an unclear effect of considered T>C polymorphism on TNF-RII gene expression in bovine leukocytes and they suggest the involvement of BLV in modifying the TNF-RII expression in BLV-infected cows potentially implying the EBL (Enzootic Bovine Leukosis) associated pathogenesis. |
| Databáze: | OpenAIRE |
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