Construction of a Transcriptome-Driven Network at the Early Stage of Infection with Influenza A H1N1 in Human Lung Alveolar Epithelial Cells
| Autor: | Young Seek Lee, Myungguen Chung, Soo Young Cho |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Apoptosis Biology Biochemistry Virus Transcriptome 03 medical and health sciences 0302 clinical medicine Drug Discovery medicine Gene Pharmacology A549 cell Regulation of gene expression Gene expression regulation RNA Sequence analysis RNA Cell biology 030104 developmental biology medicine.anatomical_structure A549 Cells 030220 oncology & carcinogenesis Influenza A virus H1N1 subtype Molecular Medicine Original Article High-throughput nucleotide sequencing |
| Zdroj: | Biomolecules & Therapeutics |
| ISSN: | 1976-9148 |
| Popis: | We aimed to understand the molecular changes in host cells that accompany infection by the seasonal influenza A H1N1 virus because the initial response rapidly changes owing to the fact that the virus has a robust initial propagation phase. Human epithelial alveolar A549 cells were infected and total RNA was extracted at 30 min, 1 h, 2 h, 4 h, 8 h, 24 h, and 48 h post infection (h.p.i.). The differentially expressed host genes were clustered into two distinct sets of genes as the infection progressed over time. The patterns of expression were significantly different at the early stages of infection. One of the responses showed roles similar to those associated with the enrichment gene sets to known 'gp120 pathway in HIV.' This gene set contains genes known to play roles in preventing the progress of apoptosis, which infected cells undergo as a response to viral infection. The other gene set showed enrichment of 'Drug Metabolism Enzymes (DMEs).' The identification of two distinct gene sets indicates that the virus regulates the cell's mechanisms to create a favorable environment for its stable replication and protection of gene metabolites within 8 h. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|