New reassortant and enzootic European swine influenza viruses transmit efficiently through direct contact in the ferret model
| Autor: | Richard J. Webby, Lars Erik Larsen, Thomas P. Fabrizio, Kristina Fobian, Sun-Woo Yoon, Mette Sif Hansen |
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| Rok vydání: | 2015 |
| Předmět: |
Swine
viruses Molecular Sequence Data Virus Attachment Virus Replication H5N1 genetic structure Virus Cell Line Orthomyxoviridae Infections Polysaccharides Virology Influenza A Virus H1N2 Subtype Reassortant Viruses Pandemic Animals Humans Swine Diseases biology Influenza A Virus H3N2 Subtype Ferrets Epithelial Cells Sequence Analysis DNA Viral Load Standard Europe Disease Models Animal Viral replication biology.protein Enzootic RNA Viral Receptors Virus Neuraminidase Viral load |
| Zdroj: | The Journal of general virology. 96(Pt 7) |
| ISSN: | 1465-2099 |
| Popis: | The reverse zoonotic events that introduced the 2009 pandemic influenza virus into pigs have drastically increased the diversity of swine influenza viruses in Europe. The pandemic potential of these novel reassortments is still unclear, necessitating enhanced surveillance of European pigs with additional focus on risk assessment of these new viruses. In this study, four European swine influenza viruses were assessed for their zoonotic potential. Two of the four viruses were enzootic viruses of subtype H1N2 (with avian-like H1) and H3N2, and two were new reassortants, one with avian-like H1 and human-like N2 and one with 2009 pandemic H1 and swine-like N2. All viruses replicated to high titres in nasal wash and nasal turbinate samples from inoculated ferrets and transmitted efficiently by direct contact. Only the H3N2 virus transmitted to naive ferrets via the airborne route. Growth kinetics using a differentiated human bronchial epithelial cell line showed that all four viruses were able to replicate to high titres. Further, the viruses revealed preferential binding to the 2,6-α-silalylated glycans and investigation of the antiviral susceptibility of the viruses revealed that all were sensitive to neuraminidase inhibitors. These findings suggested that these viruses have the potential to infect humans and further underline the need for continued surveillance as well as biological characterization of new influenza A viruses. |
| Databáze: | OpenAIRE |
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