Saliva Quantification of SARS-CoV-2 in Real-Time PCR From Asymptomatic or Mild COVID-19 Adults
| Autor: | Florence Carrouel, Emilie Gadea, Aurélie Esparcieux, Jérome Dimet, Marie Elodie Langlois, Hervé Perrier, Claude Dussart, Denis Bourgeois |
|---|---|
| Přispěvatelé: | Parcours santé systémique (P2S), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Microbiology (medical)
0303 health sciences saliva virus isolation SARS-CoV-2 infectivity COVID-19 [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Microbiology QR1-502 3. Good health viral load real-time reverse transcription PCR 03 medical and health sciences 0302 clinical medicine quantitative [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie 030212 general & internal medicine [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology 030304 developmental biology Original Research |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, 2022, ⟨10.3389/fmicb.2021.786042⟩ Frontiers in Microbiology, Vol 12 (2022) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2021.786042⟩ |
| Popis: | The fast spread of COVID-19 is related to the highly infectious nature of SARS-CoV-2. The disease is suggested to be transmitted through saliva droplets and nasal discharge. The saliva quantification of SARS-CoV-2 in real-time PCR from asymptomatic or mild COVID-19 adults has not been fully documented. This study analyzed the relationship between salivary viral load on demographics and clinical characteristics including symptoms, co-morbidities in 160 adults diagnosed as COVID-19 positive patients recruited between September and December 2020 in four French centers. Median initial viral load was 4.12 log10 copies/mL (IQR 2.95–5.16; range 0–10.19 log10 copies/mL). 68.6% of adults had no viral load detected. A median load reduction of 23% was observed between 0–2 days and 3–5 days, and of 11% between 3–5 days and 6–9 days for the delay from onset of symptoms to saliva sampling. No significant median difference between no-symptoms vs. symptoms patients was observed. Charge was consistently similar for the majority of the clinical symptoms excepted for headache with a median load value of 3.78 log10 copies/mL [1.95–4.58] (P < 0.003). SARS-CoV-2 RNA viral load was associated with headache and gastro-intestinal symptoms. The study found no statistically significant difference in viral loads between age groups, sex, or presence de co-morbidity. Our data suggest that oral cavity is an important site for SARS-CoV-2 infection and implicate saliva as a potential route of SARS-CoV-2 transmission. |
| Databáze: | OpenAIRE |
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