Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates
| Autor: | Marta Saccomanno, Jounghyun Um, Shane T. Kenny, Graham M. Hughes, Niall Beagan, Tanja Narancic, Umar Abdulmutalib, Huihai Wu, Kevin E. O’Connor, Manuel Salvador, José I. Jiménez |
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| Přispěvatelé: | Biotechnology and Biological Sciences Research Council |
| Rok vydání: | 2021 |
| Předmět: |
PHYLOGENY
Operon AROMATIC CATABOLIC PATHWAYS Bioengineering Microbiology SEQUENCE Applied Microbiology and Biotechnology Biochemistry Genome Polyhydroxyalkanoates PLASTIC WASTE 03 medical and health sciences chemistry.chemical_compound Plasmid Pseudomonas ENTNER-DOUDOROFF Research Articles 030304 developmental biology Terephthalic acid 0303 health sciences Science & Technology biology 030306 microbiology Polyethylene Terephthalates Pseudomonas putida DEGRADATION TEREPHTHALATE biology.organism_classification ALIGNMENT Biotechnology & Applied Microbiology chemistry BACTERIUM PUTIDA KT2440 Biodegradable plastic Life Sciences & Biomedicine Ethylene glycol TP248.13-248.65 0605 Microbiology Biotechnology Research Article |
| Zdroj: | Microbial Biotechnology Microbial Biotechnology, Vol 14, Iss 6, Pp 2463-2480 (2021) |
| ISSN: | 1751-7915 |
| DOI: | 10.1111/1751-7915.13712 |
| Popis: | In this article we have summarised our findings resulting from analysing the complete genome sequence of Pseudomonas umsongensis GO16 and we have determined experimentally its potential for the upcycling of monomers resulting from PET hydrolysis. Besides a general genomic characterisation, we have identified and validated the set of genes required for terephthalate and ethylene glycol degradation as well as the ability of the strain for producing short and medium‐chain length polyhydroxyalkanoates. Summary The throwaway culture related to the single‐use materials such as polyethylene terephthalate (PET) has created a major environmental concern. Recycling of PET waste into biodegradable plastic polyhydroxyalkanoate (PHA) creates an opportunity to improve resource efficiency and contribute to a circular economy. We sequenced the genome of Pseudomonas umsongensis GO16 previously shown to convert PET‐derived terephthalic acid (TA) into PHA and performed an in‐depth genome analysis. GO16 can degrade a range of aromatic substrates in addition to TA, due to the presence of a catabolic plasmid pENK22. The genetic complement required for the degradation of TA via protocatechuate was identified and its functionality was confirmed by transferring the tph operon into Pseudomonas putida KT2440, which is unable to utilize TA naturally. We also identified the genes involved in ethylene glycol (EG) metabolism, the second PET monomer, and validated the capacity of GO16 to use EG as a sole source of carbon and energy. Moreover, GO16 possesses genes for the synthesis of both medium and short chain length PHA and we have demonstrated the capacity of the strain to convert mixed TA and EG into PHA. The metabolic versatility of GO16 highlights the potential of this organism for biotransformations using PET waste as a feedstock. |
| Databáze: | OpenAIRE |
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