A Canarypox Vaccine Expressing Multiple Human Immunodeficiency Virus Type 1 Genes Given Alone or with Rgp120 Elicits Broad and Durable CD8+Cytotoxic T Lymphocyte Responses in Seronegative Volunteers
| Autor: | Mark J. Mulligan, Kent J. Weinhold, Barney S. Graham, Patricia E. Fast, David C. Montefiori, Jean-Louis Excler, Geoffrey J. Gorse, M. Juliana McElrath, Ann-Marie Duliege, Michael C. Keefer, Mary Clare Walker, Mary Lou Clements-Mann, James Tartaglia, Mark Wolff, Thomas G. Evans |
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| Rok vydání: | 1999 |
| Předmět: |
Canarypox
Genes Viral viruses T cell Genetic Vectors Retroviridae Proteins Canarypox Vector chemical and pharmacologic phenomena Canarypox virus HIV Antibodies HIV Envelope Protein gp120 Lymphocyte Activation Epitope Avipoxvirus Double-Blind Method HIV Seronegativity medicine Humans Immunology and Allergy Cytotoxic T cell Neutralizing antibody Immunization Schedule AIDS Vaccines Vaccines Synthetic biology virus diseases biology.organism_classification Virology CTL Infectious Diseases medicine.anatomical_structure Immunology HIV-1 biology.protein T-Lymphocytes Cytotoxic |
| Zdroj: | The Journal of Infectious Diseases. 180:290-298 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/314895 |
| Popis: | Induction of CD8 + cytotoxic T cells is considered one of the important correlates for the protective efficacy of candidate human immunodeficiency virus type 1 (HIV-1) vaccines. To induce CD8 + cytotoxic T lymphocytes (CTLs) along with neutralizing antibody and CD4 + T cell help, a live canarypox virus construct expressing gp120, transmembrane gp41, the gag and protease genes, and sequences containing CTL epitopes in nef and pol was given simultaneously with, or followed by, rgp120 SF2. CD8 + CTLs were detected in 61% of volunteers at some time during the trial. Three to 6 months after the last immunization, the gene-specific responses were gag, 26/81; env, 17/77; nef, 12/77; and pol, 3/16. Simultaneous immunization with the canarypox vector and the subunit, beginning with the initial immunization, resulted in earlier antibody responses. In summary, a strategy of immunization with a canarypox vector expressing multiple genes of HIV-1 given with gp120 results in durable CD8 + CTL responses to a broad range of epitopes. |
| Databáze: | OpenAIRE |
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