IgE antibody repertoire in nasal secretions of children and adults with seasonal allergic rhinitis: A molecular analysis

Autor:	Alexander Rohrbach, Stefania Arasi, Serena Perna, Salvatore Tripodi, Ilaria Simonelli, Sveva Castelli, Paolo Maria Matricardi, Mariaelisabetta Conte, Valentina Panetta, Paola Martelli, Danilo Villalta, Valeria Villella, Marco Di Fraia, Andrea Di Rienzo Businco, Ifigenia Sfika, Ekaterina Potapova, Stephanie Dramburg
Rok vydání:	2020
Předmět:	Adult Bodily Secretions Adolescent Microarray Population level diagnosis Animal food Immunology Nose medicine.disease_cause Immunoglobulin E Cohort Studies Young Adult Allergen pollen allergy Vegetables otorhinolaryngologic diseases Animals Humans Immunology and Allergy Medicine Child Aged allergic rhinitis nasal secretions biology business.industry fungi Rhinitis Allergic Seasonal Total ige Allergens Middle Aged allergen molecules Microarray Analysis Molecular analysis Pediatrics Perinatology and Child Health biology.protein Pollen immunoglobulin E microarray Antibody business 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
Zdroj:	Pediatric Allergy and Immunology. 31:273-280
ISSN:	1399-3038 0905-6157
Popis:	Background: There is growing interest both in testing IgE in nasal secretions (NS) and in molecular diagnosis of seasonal allergic rhinitis (SAR). Yet, the reliability of nasal IgE detection with the newest molecular assays has never been assessed in a large cohort of pollen allergic patients. Objective: To investigate with microarray technology and compare the repertoires of specific IgE (sIgE) antibodies in NS and sera of a large population of children and adults with SAR. Methods: Nasal secretions were collected with an absorbent device (Merocel 2000��, Medtronic) and a minimal dilution procedure from 90 children and 71 adults with SAR. Total IgE (tIgE) (ImmunoCAP, Thermo Fisher Scientific (TFS)) and sIgE antibodies against 112 allergen molecules (ISAC-112, TFS) were measured in NS and serum. Results: Nasal sIgE was detectable in 68.3% of the patients. The detected nasal sIgE antibodies recognized airborne (88%), vegetable (10%), and animal food or other (
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8d49a578b13e7a9b3933eed0ce82599 https://doi.org/10.1111/pai.13148 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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