Levels of steroidogenic acute regulatory protein and mitochondrial membrane potential in granulosa cells of older poor-responder women
| Autor: | Orly Joseph, Neri Laufer, Shveiky David, Alexander Simon, Muhammad Fatum, Yivgi-Ohana Natalie |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Aging endocrine system medicine.medical_specialty medicine.medical_treatment Fertilization in Vitro Mitochondrion Biology Membrane Potentials Young Adult Follicle Ovarian Follicle Pregnancy Internal medicine medicine Humans Cholesterol Side-Chain Cleavage Enzyme Probability Granulosa Cells In vitro fertilisation urogenital system Cholesterol side-chain cleavage enzyme Steroidogenic acute regulatory protein Obstetrics and Gynecology Embryo Transfer Phosphoproteins Abortion Spontaneous Steroid hormone Fertility Endocrinology Reproductive Medicine Apoptosis Mitochondrial Membranes Female Hormone |
| Zdroj: | Fertility and Sterility. 91:220-225 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2007.10.027 |
| Popis: | Objective To compare mitochondrial function in granulosa cells obtained from older (>40 y) low-responder IVF patients with that of young ( Design Prospective laboratory research. Setting In vitro fertilization unit in a university hospital. Patient(s) Twenty patients undergoing IVF treatment cycles. Intervention(s) Ultrasound guided oocytes pick-up. Main Outcome Measure(s) Mitochondrial function examined by using JC-1 stain for the mitochondrial membrane potential in granulosa cells of both groups and Western blots for assaying and quantification of steroidogenic acute regulatory protein (StAR) and p450scc (side-chain cleavage). Result(s) The number of granulosa cells per follicle differed between the two groups, with fewer granulosa cells isolated in the older low-responder women, compared with in the young, normal responders who were the control women. Trypan blue–negative cells showed similar undisturbed mitochondrial membrane potential, and similar ratios of apoptotic granulosa cells were observed in the two groups. In addition, there was no difference in StAR and P450scc protein levels between the two groups. Conclusion(s) Our results demonstrate a significant decrease in the number of total aspirated granulosa cells per follicle in older, poor-responder women, which probably explains the reduced hormonal production by those follicles. However, those cells demonstrate normal mitochondrial membrane potential as well as similar levels of StAR, P450scc, and de novo steroid hormone synthesis in the two groups of patients. Our results do not support mitochondrial dysfunction as a main mechanism of reproductive aging. |
| Databáze: | OpenAIRE |
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