Phenotypic characterization of encephalitis in the brains of goats experimentally infected with Spanish goat encephalitis virus
| Autor: | Ana Balseiro, Ileana Z. Martínez, Ramón A. Juste, L. M. Salinas, Claudia Pérez-Martínez, J. F. García-Marín |
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| Přispěvatelé: | CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Principado de Asturias, European Commission |
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty 040301 veterinary sciences Immunology Population Central nervous system Biology Cell population Encephalitis Viruses Tick-Borne Flavivirus Infections 0403 veterinary science Lesion 03 medical and health sciences Immune system medicine Animals education 030304 developmental biology 0303 health sciences education.field_of_study Immunity Cellular Goat Diseases General Veterinary Flavivirus Goats Brain 04 agricultural and veterinary sciences Spanish Goat Encephalitis Virus medicine.disease biology.organism_classification Spinal cord Immunohistochemistry Astrogliosis medicine.anatomical_structure Phenotype nervous system Spain Goat Encephalitis Female Microglia medicine.symptom |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 7 páginas, 2 tablas, 3 figuras. Spanish goat encephalitis virus (SGEV) is a novel tick-borne flavivirus subtype, closely related to the flavivirus louping ill virus (LIV). SGEV caused a severe, acute and mortal neurological disease outbreak in northern Spain in a goat herd. In order to characterize the cell population in lesions and to determine the distribution of the inflammatory cells, central nervous system (CNS) samples of nine female Alpine goats challenged subcutaneously with SGEV over the right thorax behind the elbow were evaluated using immunohistochemistry (microglia-Iba1, T lymphocytes-CD3, B lymphocytes-CD20 and astrocytes-GFAP). The number of microglia (37.8 %) and T lymphocytes (21.5 %) was greater than the number of B lymphocytes (16.8 %). Goats were classified into clusters based on the severity of histological lesions in CNS (A-mild to moderate lesions and B-severe lesions). Microglia was significantly more abundant than T and B lymphocytes in cluster B (severe lesions). The total area occupied by glial foci revealed that medulla oblongata and spinal cord were the most affected tissues. Astrogliosis (GFAP+) was present in the majority of the CNS sections being near to the pial surface. The lesion predominance on the right side of the medulla oblongata, which could be associated to the site of challenge suggestive of neurotropic route was also statistically confirmed. Results suggest that the cellular immune response would be the most important response to the SGEV infection. This work was partially supported by a grant from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), reference number E- RTA2013-00013-C04-04 (FEDER co-funded) and the Principado de Asturias, PCTI 2018–220 (GRUPIN: IDI2018-000237 and FEDER). Ms. Ileana Z. Martínez was supported by a Fundacion Carolina PhD scholarship (2017 call). We thank Dr. Kevin P. Dalton for critically reviewing this article. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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