Extracellular expression of Thermobifida fusca cutinase with pelB signal peptide depends on more than type II secretion pathway in Escherichia coli
| Autor: | Jing Wu, Lingang Yu, Lingqia Su, Chenhua Xu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cutinase
Signal peptide Cell Membrane Permeability Membrane permeability Bioengineering Biology medicine.disease_cause Cell Fractionation Applied Microbiology and Biotechnology Industrial Microbiology Type II Secretion Systems Extracellular medicine Escherichia coli Secretion DNA Primers Polysaccharide-Lyases Hydrolysis Phosphatidylethanolamines General Medicine Periplasmic space Fusion protein Actinobacteria Biochemistry Electrophoresis Polyacrylamide Gel Carboxylic Ester Hydrolases Biotechnology Plasmids |
| Zdroj: | Journal of biotechnology. 204 |
| ISSN: | 1873-4863 |
| Popis: | Our previous studies demonstrated that Thermobifida fusca cutinase is released into culture medium when expressed without a signal peptide in Escherichia coli, and this extracellular expression results from an enhanced membrane permeability caused by cutinase's phospholipid hydrolase activity. The present study investigated whether this phenomenon would also occur during the expression of cutinase fused to pelB signal peptide (pelB-cutinase). Secretion of fusion proteins of this type is generally believed to occur via type II secretion pathway. The results showed that when pelB-cutinase was expressed in a secB knockout strain, which has a defective type II secretion pathway, there was still a large amount of cutinase in the culture medium. Additional experiments confirmed that the periplasmic and cytoplasmic fractions of the expressing cells had hydrolytic activity toward phosphatidyl ethanolamine, and the recombinant cells showed correspondingly improved membrane permeability. All these phenomena were also observed in the parent E. coli strain. Moreover, the secretion efficiency of the inactive cutinase mutant was found to be significantly lower than that of pelB-cutinase in the parent E. coli. Based on these results, the phospholipid hydrolase activity of pelB-cutinase must play a larger role in its extracellular production than does type II secretion pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect