Cellular Source(s) of Platelet-Activating-Factor Acetylhydrolase Activity in Plasma
| Autor: | Shinichiro Okamoto, Koichiro Asano, Makiko Iwata, Kouichi Fukunaga, Kazuhiro Yamaguchi, Yasuo Ikeda, Takehiko Mori, Tetsuya Shiomi |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Cell type PAF acetylhydrolase Genotype Hematopoietic System Mutation Missense Biophysics Biology Biochemistry Phospholipases A chemistry.chemical_compound In vivo Leukocytes Humans Transplantation Homologous Platelet Activating Factor Molecular Biology Bone Marrow Transplantation DNA Primers Base Sequence Platelet-activating factor Chimera Mouth Mucosa Wild type Cell Biology Middle Aged Molecular biology Tissue Donors In vitro Transplantation Haematopoiesis Cheek chemistry 1-Alkyl-2-acetylglycerophosphocholine Esterase Immunology Female lipids (amino acids peptides and proteins) |
| Zdroj: | Biochemical and Biophysical Research Communications. 261:511-514 |
| ISSN: | 0006-291X |
| Popis: | Platelet activating factor (PAF) is immediately degraded and inactivated in the bloodstream by plasma PAF acetylhydrolase (PAF-AH). Although plasma PAF-AH-like activity was secreted in vitro from various cell types such as macrophages and hepatocytes, the exact cellular source(s) of this enzyme activity in vivo remains unclear. There is a naturally-occurring missense mutation (V279F) in the plasma PAF-AH gene in the Japanese population which results in complete loss of the enzyme activity. We analyzed 52 Japanese who had received an allogeneic bone marrow transplant and maintained donor-derived hematopoiesis. Ten recipients had chimeric plasma PAF-AH genotypes between the donor-derived peripheral blood leukocytes and the recipient-derived epithelial cells of buccal mucosa. Multiple regression analysis demonstrated that PAF-AH activity in plasma depended on the donor's genotype (standardized regression coefficient = 0.68, P < 0.0001), but not on the recipient's genotype (p = 0.48). One recipient who was a V279F homozygote in leukocytes and wild type homozygote in buccal mucosa had undetectable PAF-AH activity in plasma. We conclude that most of the PAF-AH activity in human plasma originates from hematopoietic lineage cells. |
| Databáze: | OpenAIRE |
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