The tumor immune microenvironment and its implications for clinical outcome in patients with oropharyngeal squamous cell carcinoma
| Autor: | Tomas Kazda, Tetiana Shatokhina, Marek Slávik, Dominik Gurin, Markéta Hermanová, Michal Hendrych, Marcela Vesela, Iveta Selingerová |
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| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
Stromal cell medicine.medical_treatment chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Pathology and Forensic Medicine 03 medical and health sciences Lymphocytes Tumor-Infiltrating 0302 clinical medicine Immune system PD-L1 Biopsy Tumor Microenvironment medicine Humans medicine.diagnostic_test biology Squamous Cell Carcinoma of Head and Neck Tumor-infiltrating lymphocytes business.industry 030206 dentistry Prognosis 3. Good health Radiation therapy Oropharyngeal Neoplasms Otorhinolaryngology Head and Neck Neoplasms 030220 oncology & carcinogenesis Cancer research biology.protein Periodontics Immunohistochemistry Oral Surgery business CD8 |
| Zdroj: | Journal of Oral Pathology & Medicine. 49:886-896 |
| ISSN: | 1600-0714 0904-2512 |
| Popis: | Background We examined PD-L1 expression on tumor cells (TCs) and immune cells (ICs) and density of CD3+ and CD8+ tumor-infiltrating lymphocytes (TILs) in patients with oropharyngeal squamous cell carcinoma (OPSCC) and investigated their significance on clinicopathological characteristics and clinical outcomes. Methods In a cohort of 65 patients treated by definitive intensity-modulated radiotherapy (IMRT) with curative intent, immunohistochemical analysis of PD-L1 expression on TCs and ICs, and TIL subtyping was performed on primary biopsy tumor tissues, followed by prognostic evaluation of these immune response-related parameters including classification into four tumor immune microenvironment (TIM) types. To evaluate HPV status, p16 immunohistochemistry was performed. Results Densities of CD3+ and CD8+ TILs and PD-L1 expressions on TCs and ICs were significantly higher in p16+/HPV-mediated OPSCC. Patients with high densities of stromal CD8+ TILs displayed significantly better overall survival (OS) and progression-free survival (PFS). PD-L1 expression neither on tumor cells nor on immune cells affected survival outcomes. Distribution of TIM types based on the combination of PD-L1 expression on TCs and densities of CD8+ TILs is significantly different in p16+ compared with p16- OPSCC. In type III TIM (TC-PD-L1+/low CD8+ TIL density), significantly better OS was shown in p16+ group compared with p16- OPSCC. Conclusion The prognostic and predictive role of tumor immune microenvironment was confirmed for patients with OPSCC. Combining HPV status with the evaluation of densities of CD8+ TILs and PD-L1 expression including TIM classification might be of high clinical interest and warrants further prospective evaluation. |
| Databáze: | OpenAIRE |
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