Insulin-like growth factor-binding protein-2 and -3 expression in benign human prostate epithelium, prostate intraepithelial neoplasia, and adenocarcinoma of the prostate
| Autor: | M K Tennant, R S Birnbaum, P A Twomey, J B Thrasher, S R Plymate |
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| Rok vydání: | 1996 |
| Předmět: |
PCA3
Male medicine.medical_specialty Endocrinology Diabetes and Metabolism Clinical Biochemistry In situ hybridization Biology Adenocarcinoma Biochemistry Epithelium Prostate cancer Endocrinology Prostate Internal medicine medicine Humans RNA Messenger In Situ Hybridization Aged Prostatic Intraepithelial Neoplasia Intraepithelial neoplasia Biochemistry (medical) Prostatic Neoplasms Middle Aged Prostate-Specific Antigen medicine.disease Immunohistochemistry Insulin-Like Growth Factor Binding Protein 2 medicine.anatomical_structure Insulin-Like Growth Factor Binding Protein 3 Cancer cell Keratins hormones hormone substitutes and hormone antagonists |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 81(1) |
| ISSN: | 0021-972X |
| Popis: | Insulin-like growth factor (IGF)-binding proteins (IGFBPs) modulate the activity of IGFs. In vitro human prostate epithelial cells secrete IGFBP-2 and -3. In vivo IGFBP-2 is increased, and IGFBP-3 is decreased in the serum of patients with prostate cancer. Immunohistochemistry and in situ hybridization were performed to compare the expression of IGFBP-2 and -3 in vivo in prostate tissue containing benign epithelium, high grade prostate intraepithelial neoplasia (PIN), and adenocarcinoma. Immunoreactivity and messenger ribonucleic acid (mRNA) hybridization signals for IGFBP-2 and -3 were localized to epithelial cells. IGFBP-2 immunostaining intensity was significantly increased in PIN regions compared to that in normal epithelium and was further increased in malignant cells. IGFBP-2 mRNA was also significantly increased in PIN and cancer cells. IGFBP-3 immunoreactivity was significantly increased in PIN regions compared to normal epithelium; however, IGFBP-3 protein was significantly decreased in malignant cells. IGFBP-3 mRNA remained virtually unchanged in benign epithelium, PIN, and adenocarcinoma cells. These results demonstrate that increased IGFBP-2 protein in PIN and malignant cells is probably due to increased mRNA expression. However, levels of IGFBP-3 protein may be due to pre- and/or posttranslational mechanisms, including proteolysis. The changes in IGFBP-2 and -3 protein levels in prostatic tissue are in agreement with serum changes reported in patients with prostate cancer. |
| Databáze: | OpenAIRE |
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