Physiological state of the rabbit cornea following 4 degrees C moist chamber storage
| Autor: | Michael J. Doughty |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Leak
Endothelium Bicarbonate Hydrostatic pressure Eye Banks Andrology Cornea Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Animals Lagomorpha biology Endothelium Corneal Anatomy biology.organism_classification eye diseases Sensory Systems Silicone oil Cold Temperature Ophthalmology medicine.anatomical_structure chemistry Tonicity Female sense organs Rabbits Tissue Preservation |
| Zdroj: | Experimental eye research. 49(5) |
| ISSN: | 0014-4835 |
| Popis: | Eyes from female albino rabbits (1.9-2.3 kg) were enucleated immediately following euthanasia along with the lids and conjunctiva. The globes were moist chamber-stored (in a 4 degrees C refrigerator with the lids closed and the cornea facing downwards) for 12-500 hr. Central corneal thickness (by ultrasound) increased from 350 to approximately 650 microns within 72 hr but changed little thereafter. In the latter period, the relative fluid pressure of the globe (pneumatonography) dropped to less than 10 mmHg, aqueous bicarbonate (tCO2) levels fell to less than 5 mM and anterior chamber fluid tonicity decreased progressively (especially after 24 hr storage) to reach values of around 200 mosmol l-1 by 7 days. Storma-endothelium preparations of the corneas, after 2 hr of in vitro equilibration with a bicarbonate-Ringer solution (supplemented with glucose, adenosine and glutathione) were evaluated for their ability to undergo deturgescence under silicone oil or to pump fluid against a hydrostatic pressure of 20 cmH2O. Corneal preparations from up to 7 days storage showed rapid (60 to 135 microns hr-1) and complete deturgescence (net change in thickness of 140-180 microns) that was maintained. Thereafter, the ability to show deturgescence declined to zero by 10 days. In marked contrast, endothelial fluid pump activity (of approximately 5 microliters hr-1) was manifest for only 36 hr after which time this function rapidly declined. Most corneas stored for periods longer than 48 hr exhibited a continuous leak (of -1 to -5 microliters hr-1). The results indicate that corneal deturgescence and endothelial fluid pump function are not necessarily coupled in vitro. |
| Databáze: | OpenAIRE |
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