Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate fromUlmus pumilaLeaves by GC-MS
Autor: | Bao-lin Shi, Bao-jia Gao, Zhi-hong Huang, Zhi-li Wang, Dong Wei, Jian-xin Chen, Su-li Wang |
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Rok vydání: | 2015 |
Předmět: |
lcsh:QD71-142
Methyl jasmonate Chromatography Article Subject biology Jasmonic acid lcsh:Analytical chemistry Ethyl acetate biology.organism_classification Analytical Chemistry Ulmus pumila chemistry.chemical_compound chemistry Biochemistry Amine gas treating Methanol Salicylic acid Methyl salicylate Research Article |
Zdroj: | International Journal of Analytical Chemistry International Journal of Analytical Chemistry, Vol 2015 (2015) |
ISSN: | 1687-8779 1687-8760 |
Popis: | Salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate are important phytohormones and defensive signaling compounds, so it is of great importance to determine their levels rapidly and accurately. The study usesUlmus pumilaleaves infected byTetraneura akinire Sasakiat different stages as materials; after extraction with 80% methanol and ethyl acetate and purification with primary secondary amine (PSA) and graphitized carbon blacks (GCB), the contents of signal compounds salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate were determined by GC-MS. The results showed that the level of salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate increased remarkably inU. pumilaonce infected byT. akinire Sasaki, but the maximums of these four compounds occurred at different times. Salicylic acid level reached the highest at the early stage, and jasmonic acid level went to the maximum in the middle stage; by contrast, change of content of methyl salicylate and methyl jasmonate was the quite opposite. |
Databáze: | OpenAIRE |
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