Potent antiviral effect of silver nanoparticles on SARS-CoV-2
| Autor: | Kei Miyakawa, Takeshi Morita, Sundararaj Stanleyraj Jeremiah, Yutaro Yamaoka, Akihide Ryo |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Silver Colloidal silver Cell Survival Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Pneumonia Viral Biophysics Metal Nanoparticles Antiviral Agents Biochemistry Article Silver nanoparticle Cell Line Microbiology Betacoronavirus 03 medical and health sciences 0302 clinical medicine Viral entry Chlorocebus aethiops Extracellular Animals Humans Cytotoxic T cell Luciferase Particle Size Pandemics Vero Cells Molecular Biology Dose-Response Relationship Drug SARS-CoV-2 Chemistry COVID-19 Cell Biology Virus Internalization 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Vero cell Silver nanoparticles Coronavirus Infections |
| Zdroj: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| Popis: | The pandemic of COVID-19 is spreading unchecked due to the lack of effective antiviral measures. Silver nanoparticles (AgNP) have been studied to possess antiviral properties and are presumed to inhibit SARS-CoV-2. Due to the need for an effective agent against SARS-CoV-2, we evaluated the antiviral effect of AgNPs. We evaluated a plethora of AgNPs of different sizes and concentration and observed that particles of diameter around 10 nm were effective in inhibiting extracellular SARS-CoV-2 at concentrations ranging between 1 and 10 ppm while cytotoxic effect was observed at concentrations of 20 ppm and above. Luciferase-based pseudovirus entry assay revealed that AgNPs potently inhibited viral entry step via disrupting viral integrity. These results indicate that AgNPs are highly potent microbicides against SARS-CoV-2 but should be used with caution due to their cytotoxic effects and their potential to derange environmental ecosystems when improperly disposed. Highlights • Silver nanoparticles inhibit extracellular SARS-CoV-2. • 2–15 nm size particles showed robust inhibition of SARS-CoV-2. • SARS-CoV-2 was potently inhibited at concentrations above 1 ppm. • Naked Silver nanoparticles exhibited cytotoxicity from 20 ppm onwards. |
| Databáze: | OpenAIRE |
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