| Popis: |
A procedure was developed for the purification and concentration of Mahoney, MEF-1, and Saukett strains of poliomyelitis virus from large volumes of tissue culture fluid. The basic procedure involved precipitation, butanol extraction, ultracentrifugation, and treatment with nucleases. Final separation of virus particles from residual cellular contaminants was achieved by electrophoresis or sedimentation in a density gradient. From tissue culture fluid in which the virus represented approximately 0.1% of the total protein, preparations of virus particles, some of which were crystalline, were obtained which closely approached chemical purity. Such preparations make possible studies of the serological, chemical, and physical properties of the virus. |
