Galanin: a novel intraovarian regulatory peptide
| Autor: | Samuel C. Keeble, Georgette Howard, Kenneth N. Muse, James F. Hyde, Thomas E. Curry, Steve N. London, M D Fox |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Granulosa cell Neuropeptide Galanin Ovary Biology Chorionic Gonadotropin Rats Sprague-Dawley Paracrine signalling Endocrinology Internal medicine medicine Animals RNA Messenger Cells Cultured Progesterone Estradiol digestive oral and skin physiology Metalloendopeptidases Luteinizing Hormone Rats medicine.anatomical_structure Gene Expression Regulation nervous system Theca Culture Media Conditioned Solution hybridization Female Gonadotropin Peptides hormones hormone substitutes and hormone antagonists |
| Zdroj: | Endocrinology. 135:636-641 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.135.2.7518387 |
| Popis: | Galanin is a 29-amino acid peptide that acts as a neuropeptide in many tissues. To date, galanin action and the hormonal regulation of galanin gene expression have not been described in the ovary of any species. To study possible ovarian expression and regulation of galanin, immature gonadotropin-primed rats were given hCG (10 IU), and their ovaries were collected 0, 4, 8, 12, and 20 h after hCG treatment for determination of galanin messenger RNA (mRNA) concentration by solution hybridization. Galanin mRNA levels progressively increased after hCG administration, peaking at 12 h (2.4-fold increase vs. 0 h), with a subsequent return to 0 h levels at 20 h. To determine a possible ovarian role for galanin, rats were killed 48 h after gonadotropin administration, their ovaries were removed, and granulosa cells were harvested. These cells and the ovarian tissue remaining after granulosa cell collection (i.e. "shells") were each cultured for 24 h with increasing concentrations of galanin (0, 10, 100, and 1000 nM) in the presence or absence of LH. The medium was examined for steroid production and metalloproteinase inhibitor activity. In granulosa cell cultures, galanin increased the levels of estradiol by 26% and had no effect on progesterone, but decreased metalloproteinase inhibitor activity by 61% in the conditioned medium. In the shell cultures, galanin increased estradiol, progesterone, and androstenedione in the medium, suggesting that galanin acts on cells other than granulosa cells or that galanin action requires a paracrine interaction between granulosa and thecal cells. Our data demonstrate that galanin message is increased by hCG, and that galanin acts to amplify ovarian steroidogenesis while decreasing metalloproteinase inhibitor activity. These findings establish that ovarian galanin mRNA is hormonally stimulated and that galanin acts as an intraovarian regulatory peptide. |
| Databáze: | OpenAIRE |
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