Priming effect of met-enkephalin and β-endorphin on chemiluminescence, chemotaxis and CD11b molecule expression on human neutrophils in vitro
| Autor: | Henryk Tchórzewski, Marcin Tchórzewski, Jarek Paśnik, Zbigniew Baj, Marek Luciak |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Met-enkephalin
endocrine system medicine.medical_specialty Enkephalin Neutrophils Enkephalin Methionine Immunology Integrin alphaXbeta2 Macrophage-1 Antigen Proinflammatory cytokine Mice chemistry.chemical_compound In vivo Internal medicine medicine Animals Humans Immunology and Allergy Opioid peptide Cells Cultured biology Chemotaxis Receptors IgG beta-Endorphin hemic and immune systems Flow Cytometry Lymphocyte Function-Associated Antigen-1 Endocrinology chemistry Integrin alpha M CD18 Antigens Luminescent Measurements biology.protein hormones hormone substitutes and hormone antagonists |
| Zdroj: | Immunology Letters. 67:77-83 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/s0165-2478(98)00114-x |
| Popis: | The opioid peptides are widely distributed throughout the body, and they are generated during stress and inflammatory reaction. Opioids are involved in the communication between the immune and neuroendocrine systems. In the present study we have investigated the ability of both met-enkephalin and beta-endorphin to stimulate and prime the human neutrophils for enhanced chemiluminescence (CL) and chemotaxis induced with fMLP, OZ or PMA. We have also tested the effect of beta-endorphin and met-enkephalin on CD11a, CD11b, CD18 and CD16 molecule expression on PMN in vitro. PMN from ten healthy donors were incubated in vitro with different concentrations of beta-endorphin or met-enkephalin, and the CL response was evaluated with luminometer. To assess the effect of opioid peptides on CD11a, CD11b, CD18 and CD16 molecule expression the whole blood samples were incubated with different concentrations of the opioids, then the white cells were labelled with respective PE-conjugated MoAb and evaluated by flow cytometry. We have shown that: (1) met-enkephalin and beta-endorphin at physiological concentrations relevant to that of in vivo (10(-8) and 10(-6) M) enhanced fMLP, PMA or OZ stimulated chemiluminescence and induced chemotactic response, (2) High concentrations of beta-endorphin (10(-3) M) or met-enkephalin (10(-5) M) decreased the CL response of PMN in vitro, (3) The opioid peptides at lower concentrations resulted in CD11b and CD18 molecule up-regulation on neutrophils. We may conclude that opioid peptides in physiological concentration are involved in neutrophil priming whereas in higher concentration exert immunosuppressive potency. Opioid peptides like inflammatory cytokines may prime the neutrophils inflammatory response. |
| Databáze: | OpenAIRE |
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