Variation in seed longevity among diverse Indica rice varieties

Autor: Pompe C. Sta. Cruz, Rocel Valdez, Abdel M. Ismail, Tobias Kretzschmar, Jae-Sung Lee, Myrish Pacleb, Marlina Velasco-Punzalan, Kenneth L. McNally, N. Ruaraidh Sackville Hamilton, Fiona R. Hay
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Annals of Botany
Lee, J-S, Velasco-Punzalan, M, Pacleb, M, Valdez, R, Kretzschmar, T, McNally, K, Ismail, A, Sta. Cruz, P, Sackville Hamilton, N R & Hay, F R 2019, ' Variation in seed longevity among diverse Indica rice varieties ', Annals of Botany, vol. 124, pp. 447-460 . https://doi.org/10.1093/aob/mcz093
Lee, J S, Velasco-Punzalan, M, Pacleb, M, Valdez, R, Kretzschmar, T, McNally, K L, Ismail, A M, Cruz, P C S, Sackville Hamilton, N R & Hay, F R 2019, ' Variation in seed longevity among diverse Indica rice varieties ', Annals of botany, vol. 124, no. 3, pp. 447-460 . https://doi.org/10.1093/aob/mcz093
ISSN: 1095-8290
0305-7364
DOI: 10.1093/aob/mcz093
Popis: Background and Aims Understanding variation in seed longevity, especially within closely related germplasm, will lead to better understanding of the molecular basis of this trait, which is particularly important for seed genebanks, but is also relevant to anyone handling seeds. We therefore set out to determine the relative seed longevity of diverse Indica rice accessions through storage experiments. Since antioxidants are purported to play a role in seed storability, the antioxidant activity and phenolic content of caryopses were determined. Methods Seeds of 299 Indica rice accessions harvested at 31, 38 and 45 d after heading (DAH) between March and May 2015 and differing in harvest moisture content (MC) were subsequently stored at 10.9 % MC and 45 °C. Samples were taken at regular intervals and sown for germination. Germination data were subjected to probit analysis and the resulting parameters that describe the loss of viability during storage were used for genome-wide association (GWA) analysis. Key Results The seed longevity parameters, Ki [initial viability in normal equivalent deviates (NED)], −σ−1 (σ is the time for viability to fall by 1 NED in experimental storage) and p50 [time for viability to fall to 50 % (0 NED)], varied considerably across the 299 Indica accessions. Seed longevity tended to increase as harvest MC decreased and to decrease as harvest MC increased. Eight major loci associated with seed longevity parameters were identified through GWA analysis. The favourable haplotypes on chromosomes 1, 3, 4, 9 and 11 enhanced p50 by ratios of 0.22–1.86. Conclusions This is the first study to describe the extent of variation in σ within a species’ variety group. A priori candidate genes selected based on rice genome annotation and gene network ontology databases suggested that the mechanisms conferring high seed longevity might be related to DNA repair and transcription, sugar metabolism, reactive oxygen species scavenging and embryonic/root development.
Databáze: OpenAIRE
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