Expression of genes involved in fatty acid transport and insulin signaling is altered by physical inactivity and exercise training in human skeletal muscle
| Autor: | Noortje T. L. van Duijnhoven, Fleur Poelkens, Elisabeth M. Pardoel, Maria T. E. Hopman, Dick H. J. Thijssen, Gerwen Lammers, Joost G. J. Hoenderop |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Health aging / healthy living [IGMD 5] Physiology Biopsy Endocrinology Diabetes and Metabolism medicine.medical_treatment Fatty Acid-Binding Proteins Young Adult chemistry.chemical_compound Insulin resistance Adipokines Deconditioning Downregulation and upregulation Physiology (medical) Internal medicine medicine Humans Insulin Muscle Skeletal Exercise Spinal Cord Injuries Oligonucleotide Array Sequence Analysis chemistry.chemical_classification biology Fatty acid metabolism Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling 3-Hydroxyacyl CoA Dehydrogenases Membrane Transport Proteins Fatty acid medicine.disease Insulin receptor Human Reproduction [NCEBP 12] Endocrinology Membrane transport and intracellular motility Renal disorder [NCMLS 5] Gene Expression Regulation chemistry Case-Control Studies biology.protein Physical deconditioning RNA Cardiovascular diseases Health aging / healthy living [NCEBP 14] Signal Transduction |
| Zdroj: | American Journal of Physiology : Endocrinology and Metabolism, 303, 10, pp. E1245-51 American Journal of Physiology : Endocrinology and Metabolism, 303, E1245-51 |
| ISSN: | 0193-1849 |
| Popis: | Item does not contain fulltext Physical deconditioning is associated with the development of chronic diseases, including type 2 diabetes and cardiovascular disease. Exercise training effectively counteracts these developments, but the underlying mechanisms are largely unknown. To gain more insight into these mechanisms, muscular gene expression levels were assessed after physical deconditioning and after exercise training of the lower limbs in humans by use of gene expression microarrays. To exclude systemic effects, we used human models for local physical inactivity (3 wk of unilateral limb suspension) and for local exercise training (6 wk of functional electrical stimulation exercise of the extremely deconditioned legs of individuals with a spinal cord injury). The most interesting subset of genes, those downregulated after deconditioning as well as upregulated after exercise training, contained 18 genes related to both the "insulin action" and "adipocytokine signaling" pathway. Of these genes, the three with strongest up/downregulation were the muscular fatty acid-binding protein-3 (FABP3), the fatty acid oxidizing enzyme hydroxyacyl-CoA dehydrogenase (HADH), and the mitochondrial fatty acid transporter solute carrier 25 family member A20 (SLC25A20). The expression levels of these genes were confirmed using RT-qPCR. The results of the present study indicate an important role for a decreased transport and metabolism of fatty acids, which provides a link between physical activity levels and insulin signaling. |
| Databáze: | OpenAIRE |
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