Radioimmunoassay of Hepatitis B e-antigen (HBeAg): identification of HBeAg not associated with Immunoglobulins
| Autor: | E. J. Harley, N. Strick, C. E. Stevens, A. R. Neurath, W. Szmuness |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Immunodiffusion
viruses Radioimmunoassay medicine.disease_cause Immunoglobulin G Hepatitis B Antigens Viral Proteins Blood serum Virology medicine Humans Hepatitis B virus biology Isoelectric focusing virus diseases Hepatitis B medicine.disease Molecular biology digestive system diseases Molecular Weight HBeAg biology.protein Peptides |
| Zdroj: | Journal of General Virology. 42:493-504 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-42-3-493 |
| Popis: | A radioimmunoassay for hepatitis B e-antigen (HBeAg) is described. Polystyrene beads coated with IgG prepared from a human serum containing antibodies to HBeAg (anti-HBe) and anti-HBe IgG labelled with 125I-p-hydroxyphenylpropionic acid N-hydroxysuccinimide ester were used in the test. The radioimmunoassay was approximately 1,000-fold more sensitive than immunodiffusion. At least a transient presence of HBeAg in serum appears to be a common feature of infections by hepatitis B virus. The radioimmunoassay was instrumental in establishing conditions for identification of apparently free monomeric HBeAg. The HBeAg has an approximate mol. wt.of 35,000 and was recovered after isoelectric focusing in fractions with a pH between 4.25 and 4.8. Polyacrylamide gel electrophoresis revealed the presence in HBeAg of a polypeptide with an apparent mol. wt. of 17,000. |
| Databáze: | OpenAIRE |
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