Regulation of proto-oncogene expression in adult and developing lungs
| Autor: | Tom Curran, Richard J. Smeyne, James I. Morgan, R Molinar-Rode |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Genetically modified mouse Aging Programmed cell death JUNB Restriction Mapping Gestational Age Mice Transgenic In situ hybridization Biology Mice Genes jun Gene expression Animals RNA Messenger Lung Molecular Biology In Situ Hybridization Regulation of gene expression Mice Inbred BALB C Oncogene Brain Genes fos Adrenalectomy Cell Biology beta-Galactosidase Molecular biology Cell biology Gene Expression Regulation Organ Specificity Female Proto-Oncogene Proteins c-fos Immediate early gene Research Article |
| Zdroj: | Molecular and Cellular Biology. 13:3213-3220 |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.13.6.3213 |
| Popis: | Activation of immediate-early gene expression has been associated with mitogenesis, differentiation, nerve cell depolarization, and recently, terminal differentiation processes and programmed cell death. Previous evidence also suggested that immediate-early genes play a role in the physiology of the lungs (J. I. Morgan, D. R. Cohen, J. L. Hempstead, and T. Curran, Science 237:192-197, 1987). Therefore, we analyzed c-fos expression in adult and developing lung tissues. Seizures elicited by chemoconvulsants induced expression of mRNA for c-fos, c-jun, and junB and Fos-like immunoreactivity in lung tissue. The use of pharmacological antagonists and adrenalectomy indicated that this increased expression was neurogenic. Interestingly, by using a fos-lacZ transgenic mouse, it was shown that Fos-LacZ expression in response to seizure occurred preferentially in clusters of epithelial cells at the poles of the bronchioles. This was the same location of Fos-LacZ expression detected during early lung development. These data imply that pharmacological induction of immediate-early gene expression in adult mice recapitulates an embryological program of gene expression. |
| Databáze: | OpenAIRE |
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