The Orphan Receptor CRF2-4 Is an Essential Subunit of the Interleukin 10 Receptor

Autor: Sharon Pitts-Meek, Francesco Di Marco, Michel Aguet, Bernard Sordat, Susan D. Spencer, Jeff Hooley, Anne M. Ryan, Michele Bauer, Verna C. Gibbs
Rok vydání: 1998
Předmět:
Protein Conformation
Immunology
Interleukin 5 receptor alpha subunit
Cell Separation
Interleukin 1 receptor
type II
Biology
Transfection
Article
Interleukin 10 receptor
alpha subunit
Animals
Cells
Cultured
Colitis/immunology
Flow Cytometry
Interferon-alpha/pharmacology
Interferon-gamma/pharmacology
Interleukin-10 Receptor beta Subunit
Lymphocytes/drug effects
Lymphocytes/immunology
Macrophages/drug effects
Macrophages/immunology
Membrane Glycoproteins
Mice
Mice
Inbred C57BL
Receptors
Cytokine/genetics
Receptors
Cytokine/immunology
Receptors
Interleukin/genetics
Receptors
Interleukin/immunology
Receptors
Interleukin-10
Splenomegaly/immunology
Stem Cells/drug effects
Stem Cells/immunology
Interferon-gamma
03 medical and health sciences
0302 clinical medicine
Interleukin-4 receptor
Immunology and Allergy
Lymphocytes
Receptors
Cytokine
Interleukin 12 receptor
beta 1 subunit
030304 developmental biology
Orphan receptor
0303 health sciences
Macrophages
Stem Cells
Interferon-alpha
Receptors
Interleukin
Articles
Colitis
Molecular biology
Interleukin-21 receptor
Splenomegaly
Interleukin 1 receptor
type I
030215 immunology
Zdroj: Journal of Experimental Medicine, vol. 187, no. 4, pp. 571-578
The Journal of Experimental Medicine
ISSN: 1540-9538
0022-1007
Popis: The orphan receptor CRF2-4 is a member of the class II cytokine receptor family (CRF2), which includes the interferon receptors, the interleukin (IL) 10 receptor, and tissue factor. CRFB4, the gene encoding CRF2-4, is located within a gene cluster on human chromosome 21 that comprises three interferon receptor subunits. To elucidate the role of CRF2-4, we disrupted the CRFB4 gene in mice by means of homologous recombination. Mice lacking CRF2-4 show no overt abnormalities, grow normally, and are fertile. CRF2-4 deficient cells are normally responsive to type I and type II interferons, but lack responsiveness to IL-10. By ∼12 wk of age, the majority of mutant mice raised in a conventional facility developed a chronic colitis and splenomegaly. Thus, CRFB4 mutant mice recapitulate the phenotype of IL-10–deficient mice. These findings suggest that CRF2-4 is essential for IL-10–mediated effects and is a subunit of the IL-10 receptor.
Databáze: OpenAIRE
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